转运抑制剂结核放线菌素与核酸结合，并阻断50S亚基的体外组装。
The translocation inhibitor tuberactinomycin binds to nucleic acids and blocks the in vitro assembly of 50S subunits.
作者信息
Yamada T, Teshima T, Shiba T, Nierhaus K H
出版信息
Nucleic Acids Res. 1980 Dec 11;8(23):5767-77. doi: 10.1093/nar/8.23.5767.
Binding studies were performed with a [14C]-labelled derivative of viomycin, tuberactinomycin 0 (TUM O). TUM O bound to 30S and 50S subunits. The binding component was the RNA, since ribosomal proteins did not bind the drug. Other RNAs such as tRNA, phage RNA (MS2), and homopolynucleotides also bound the drug. Striking differences in the binding capacity of the various homopolynucleotides were found. Poly(U) bound strongly, poly(G) and poly(C) bound intermediately, whereas poly(A) showed a very low binding. DNA also bound TUM O, although with native DNA the binding was only weak. Finally the effects of viomycin on the assembly in vitro of the 50S subunit from E. coli were tested. A very strong inhibition was found: when the reconstitution was performed at 0.5 x 10(-6) M viomycin the particles formed sedimented at about 50S, but showed a residual activity of less than 10%. The inhibitory power of viomycin with respect to the in vitro assembly is more pronounced than that found in in vitro systems for protein synthesis.
用[14C]标记的紫霉素衍生物结核放线菌素0（TUM O）进行结合研究。TUM O与30S和50S亚基结合。结合成分是RNA，因为核糖体蛋白不与该药物结合。其他RNA如tRNA、噬菌体RNA（MS2）和同聚核苷酸也能结合该药物。发现各种同聚核苷酸的结合能力存在显著差异。聚（U）结合力强，聚（G）和聚（C）结合力中等，而聚（A）的结合力非常低。DNA也能结合TUM O，不过与天然DNA的结合较弱。最后测试了紫霉素对大肠杆菌50S亚基体外组装的影响。发现有很强的抑制作用：当在0.5×10（-6）M紫霉素存在下进行重组时，形成的颗粒沉降在约50S，但残余活性小于10%。紫霉素对体外组装的抑制能力比在体外蛋白质合成系统中发现的更为明显。
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