培养的血管内皮细胞和平滑肌细胞外切酶对腺嘌呤核苷酸的代谢
Metabolism of adenine nucleotides by ectoenzymes of vascular endothelial and smooth-muscle cells in culture.
作者信息
Pearson J D, Carleton J S, Gordon J L
出版信息
Biochem J. 1980 Aug 15;190(2):421-9. doi: 10.1042/bj1900421.
Abstract
- Pig aortic endothelial and smooth-muscle cells in culture rapidly catabolize exogenous ATP, ADP or AMP. 2. In both cell types catabolism is due to Mg2+-stimulated ectoenzymes. 3. Inhibition and substrate-specificity studies suggest that both cell types possess three distinct ectonucleotidases, namely nucleoside triphosphatase (EC 3.6.1.15), nucleoside diphosphatase (EC 3.6.1.6) and 5'-nucleotidase (EC 3.1.3.5), as well as nucleoside diphosphate kinase (EC 2.7.4.6). 4. These ectonucleotidase systems could be of importance in the regulation of neurotransmission, blood platelet function and vasodilation.
摘要
- 培养的猪主动脉内皮细胞和平滑肌细胞能迅速分解外源性ATP、ADP或AMP。2. 在这两种细胞类型中，分解代谢是由镁离子刺激的胞外酶引起的。3. 抑制和底物特异性研究表明，这两种细胞类型都拥有三种不同的胞外核苷酸酶，即核苷三磷酸酶（EC 3.6.1.15）、核苷二磷酸酶（EC 3.6.1.6）和5'-核苷酸酶（EC 3.1.3.5），以及核苷二磷酸激酶（EC 2.7.4.6）。4. 这些胞外核苷酸酶系统可能在神经传递、血小板功能和血管舒张的调节中具有重要意义。
相似文献
1
Metabolism of adenine nucleotides by ectoenzymes of vascular endothelial and smooth-muscle cells in culture.
Biochem J. 1980 Aug 15;190(2):421-9. doi: 10.1042/bj1900421.
2
Stereoselectivity of ectonucleotidases on vascular endothelial cells.
Biochem J. 1983 Sep 15;214(3):975-81. doi: 10.1042/bj2140975.
3
Characterization of ectonucleotidases on vascular smooth-muscle cells.
Biochem J. 1985 Sep 1;230(2):503-7. doi: 10.1042/bj2300503.
4
Investigation of the preferred Mg(II)-adenine-nucleotide complex at the active site of ectonucleotidases in intact vascular cells using phosphorothioate analogues of ADP and ATP.
Eur J Biochem. 1985 Sep 2;151(2):373-5. doi: 10.1111/j.1432-1033.1985.tb09111.x.
5
Ectonucleotidase activities associated with cholinergic synaptosomes isolated from Torpedo electric organ.
J Neurochem. 1986 Sep;47(3):871-81. doi: 10.1111/j.1471-4159.1986.tb00692.x.
6
The stepwise hydrolysis of adenine nucleotides by ectoenzymes of rat renal brush-border membranes.
Biochim Biophys Acta. 1990 Nov 30;1030(1):143-51. doi: 10.1016/0005-2736(90)90249-n.
7
Enzyme kinetics and pharmacological characterization of nucleotidases released from the guinea pig isolated vas deferens during nerve stimulation: evidence for a soluble ecto-nucleoside triphosphate diphosphohydrolase-like ATPase and a soluble ecto-5'-nucleotidase-like AMPase.
J Pharmacol Exp Ther. 2002 Sep;302(3):992-1001. doi: 10.1124/jpet.102.033332.
8
ATP diphosphohydrolase is responsible for ecto-ATPase and ecto-ADPase activities in bovine aorta endothelial and smooth muscle cells.
Biochem Biophys Res Commun. 1991 Nov 14;180(3):1200-6. doi: 10.1016/s0006-291x(05)81323-3.
9
Binding of nucleotides to nucleoside diphosphate kinase monitored by rose Bengal.
Eur J Biochem. 1986 Jul 15;158(2):239-43. doi: 10.1111/j.1432-1033.1986.tb09743.x.
10
Extracellular ATP formation on vascular endothelial cells is mediated by ecto-nucleotide kinase activities via phosphotransfer reactions.
FASEB J. 2001 Jan;15(1):251-260. doi: 10.1096/fj.00-0268com.
引用本文的文献
1
Vascular smooth muscle cell dysfunction in neurodegeneration.
Front Neurosci. 2022 Nov 10;16:1010164. doi: 10.3389/fnins.2022.1010164. eCollection 2022.
2
Apyrase with anti-platelet aggregation activity from the nymph of the camel tick Hyalomma dromedarii.
Exp Appl Acarol. 2020 Mar;80(3):349-361. doi: 10.1007/s10493-020-00471-9. Epub 2020 Jan 11.
3
The Second Gaddum Lecture: its origins and outcomes.
Br J Pharmacol. 2020 Mar;177(5):969-977. doi: 10.1111/bph.14928. Epub 2020 Feb 5.
4
Vascular Endothelial Cell Biology: An Update.
Int J Mol Sci. 2019 Sep 7;20(18):4411. doi: 10.3390/ijms20184411.
5
The ecto-enzymes CD73 and adenosine deaminase modulate 5'-AMP-derived adenosine in myofibroblasts of the rat small intestine.
Purinergic Signal. 2018 Dec;14(4):409-421. doi: 10.1007/s11302-018-9623-6. Epub 2018 Sep 29.
6
Comparative hemodynamic effects of hypotension induced by diadenosine tetraphosphate (APA) and ATP in dogs.
J Anesth. 1997 Mar;11(1):44-49. doi: 10.1007/BF02480004.
7
Heterogeneous Vascular Bed Responses to Pulmonary Titanium Dioxide Nanoparticle Exposure.
Front Cardiovasc Med. 2017 May 24;4:33. doi: 10.3389/fcvm.2017.00033. eCollection 2017.
8
Integrating organ-specific function with the microcirculation.
Curr Opin Chem Eng. 2014 Feb 1;3:103-111. doi: 10.1016/j.coche.2013.12.004.
9
Sources of intravascular ATP during exercise in humans: critical role for skeletal muscle perfusion.
Exp Physiol. 2013 May;98(5):988-98. doi: 10.1113/expphysiol.2012.071555. Epub 2013 Jan 11.
10
Mechanisms of ATP release and signalling in the blood vessel wall.
Cardiovasc Res. 2012 Aug 1;95(3):269-80. doi: 10.1093/cvr/cvs187. Epub 2012 Jun 7.
本文引用的文献
1
Plaque production by the polyoma virus.
Virology. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1.
2
Plaque formation and isolation of pure lines with poliomyelitis viruses.
J Exp Med. 1954 Feb;99(2):167-82. doi: 10.1084/jem.99.2.167.
3
Disappearance of adenosine diphosphate in vivo.
J Lab Clin Med. 1969 Jan;73(1):54-9.
4
The release of nucleotides, 5-hydroxytryptamine and enzymes from human blood platelets during aggregation.
J Physiol. 1968 Apr;195(3):715-29. doi: 10.1113/jphysiol.1968.sp008484.
5
Catabolism of adenine nucleotides by the isolated perfused rat heart.
Proc Soc Exp Biol Med. 1968 Jan;127(1):33-6. doi: 10.3181/00379727-127-32614.
6
Metabolism of ( 14 C)adenine and derivatives by cerebral tissues, superfused and electrically stimulated.
Biochem J. 1972 Feb;126(4):965-73. doi: 10.1042/bj1260965.
7
Quantitative thin-layer chromatography of ATP and the products of its degradation in meat tissue.
J Chromatogr. 1974 Mar 13;90(1):105-11. doi: 10.1016/s0021-9673(01)94779-x.
8
Sites of adenosine production in cardiac and skeletal muscle.
Am J Physiol. 1973 Oct;225(4):938-53. doi: 10.1152/ajplegacy.1973.225.4.938.
9
The effects of dipyridamole on coronary post-occlusion hyperaemia and on myocardial vasodilatation induced by systemic hypoxia.
Br J Pharmacol. 1972 Dec;46(4):594-601. doi: 10.1111/j.1476-5381.1972.tb06886.x.
10
Adenylate metabolism and adenosine formation in the heart.
Am J Physiol. 1972 Nov;223(5):1119-27. doi: 10.1152/ajplegacy.1972.223.5.1119.
Zotero 插件