培养的血管内皮细胞和平滑肌细胞外切酶对腺嘌呤核苷酸的代谢
Metabolism of adenine nucleotides by ectoenzymes of vascular endothelial and smooth-muscle cells in culture.
作者信息
Pearson J D, Carleton J S, Gordon J L
出版信息
Biochem J. 1980 Aug 15;190(2):421-9. doi: 10.1042/bj1900421.
Abstract
- Pig aortic endothelial and smooth-muscle cells in culture rapidly catabolize exogenous ATP, ADP or AMP. 2. In both cell types catabolism is due to Mg2+-stimulated ectoenzymes. 3. Inhibition and substrate-specificity studies suggest that both cell types possess three distinct ectonucleotidases, namely nucleoside triphosphatase (EC 3.6.1.15), nucleoside diphosphatase (EC 3.6.1.6) and 5'-nucleotidase (EC 3.1.3.5), as well as nucleoside diphosphate kinase (EC 2.7.4.6). 4. These ectonucleotidase systems could be of importance in the regulation of neurotransmission, blood platelet function and vasodilation.
摘要
- 培养的猪主动脉内皮细胞和平滑肌细胞能迅速分解外源性ATP、ADP或AMP。2. 在这两种细胞类型中，分解代谢是由镁离子刺激的胞外酶引起的。3. 抑制和底物特异性研究表明，这两种细胞类型都拥有三种不同的胞外核苷酸酶，即核苷三磷酸酶（EC 3.6.1.15）、核苷二磷酸酶（EC 3.6.1.6）和5'-核苷酸酶（EC 3.1.3.5），以及核苷二磷酸激酶（EC 2.7.4.6）。4. 这些胞外核苷酸酶系统可能在神经传递、血小板功能和血管舒张的调节中具有重要意义。
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