培养的血管内皮细胞和平滑肌细胞外切酶对腺嘌呤核苷酸的代谢

Pearson J D, Carleton J S, Gordon J L

Biochem J. 1980 Aug 15;190(2):421-9. doi: 10.1042/bj1900421.

Pig aortic endothelial and smooth-muscle cells in culture rapidly catabolize exogenous ATP, ADP or AMP. 2. In both cell types catabolism is due to Mg2+-stimulated ectoenzymes. 3. Inhibition and substrate-specificity studies suggest that both cell types possess three distinct ectonucleotidases, namely nucleoside triphosphatase (EC 3.6.1.15), nucleoside diphosphatase (EC 3.6.1.6) and 5'-nucleotidase (EC 3.1.3.5), as well as nucleoside diphosphate kinase (EC 2.7.4.6). 4. These ectonucleotidase systems could be of importance in the regulation of neurotransmission, blood platelet function and vasodilation.

培养的猪主动脉内皮细胞和平滑肌细胞能迅速分解外源性ATP、ADP或AMP。2. 在这两种细胞类型中，分解代谢是由镁离子刺激的胞外酶引起的。3. 抑制和底物特异性研究表明，这两种细胞类型都拥有三种不同的胞外核苷酸酶，即核苷三磷酸酶（EC 3.6.1.15）、核苷二磷酸酶（EC 3.6.1.6）和5'-核苷酸酶（EC 3.1.3.5），以及核苷二磷酸激酶（EC 2.7.4.6）。4. 这些胞外核苷酸酶系统可能在神经传递、血小板功能和血管舒张的调节中具有重要意义。

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核心技术专利：CN118964589B侵权必究

Pearson J D, Carleton J S, Gordon J L

Biochem J. 1980 Aug 15;190(2):421-9. doi: 10.1042/bj1900421.

Pig aortic endothelial and smooth-muscle cells in culture rapidly catabolize exogenous ATP, ADP or AMP. 2. In both cell types catabolism is due to Mg2+-stimulated ectoenzymes. 3. Inhibition and substrate-specificity studies suggest that both cell types possess three distinct ectonucleotidases, namely nucleoside triphosphatase (EC 3.6.1.15), nucleoside diphosphatase (EC 3.6.1.6) and 5'-nucleotidase (EC 3.1.3.5), as well as nucleoside diphosphate kinase (EC 2.7.4.6). 4. These ectonucleotidase systems could be of importance in the regulation of neurotransmission, blood platelet function and vasodilation.

培养的猪主动脉内皮细胞和平滑肌细胞能迅速分解外源性ATP、ADP或AMP。2. 在这两种细胞类型中，分解代谢是由镁离子刺激的胞外酶引起的。3. 抑制和底物特异性研究表明，这两种细胞类型都拥有三种不同的胞外核苷酸酶，即核苷三磷酸酶（EC 3.6.1.15）、核苷二磷酸酶（EC 3.6.1.6）和5'-核苷酸酶（EC 3.1.3.5），以及核苷二磷酸激酶（EC 2.7.4.6）。4. 这些胞外核苷酸酶系统可能在神经传递、血小板功能和血管舒张的调节中具有重要意义。