Ardeshir F, Ames G F
J Supramol Struct. 1980;13(1):117-30. doi: 10.1002/jss.400130111.
The genes for the well-characterized high-affinity histidine transport system of S typhimurium have been cloned in lambda gt4. Genetic and physiological analyses of the analogous transport system of E coli were undertaken in order that available lambda vectors, recombinant DNA techniques, and a genetic selection for transport function might be used to isolate the Salmonella genes. The presence of the transport genes on a 12.4 Kb cloned DNA fragment has been confirmed 1) genetically, by complementation studies; 2) physiologically, by the rates of histidine uptake by bacteria containing this DNA; and 3) by demonstrating that the cloned DNA codes for the previously identified transport proteins J and P. The isolated fragment carries the entire transport operon, the argT gene and the ubiX locus, but neither the purF gene nor the ack/pta loci.
鼠伤寒沙门氏菌中特征明确的高亲和力组氨酸转运系统的基因已被克隆到λgt4中。对大肠杆菌类似转运系统进行了遗传和生理学分析，以便利用现有的λ载体、重组DNA技术以及针对转运功能的遗传筛选来分离沙门氏菌基因。已通过以下方式证实了12.4 Kb克隆DNA片段上存在转运基因：1）通过互补研究进行遗传学证实；2）通过含有该DNA的细菌摄取组氨酸的速率进行生理学证实；3）通过证明克隆的DNA编码先前鉴定的转运蛋白J和P。分离出的片段携带整个转运操纵子、argT基因和ubiX位点，但不携带purF基因或ack/pta位点。
