Goodman Y E, Wort A J, Jackson F L
J Clin Microbiol. 1981 Feb;13(2):286-92. doi: 10.1128/jcm.13.2.286-292.1981.
An enzyme-linked immunosorbent assay was developed for detection of immunoglobulin A (IgA) antibody to Bordetella pertussis (PsIgA) in nasopharyngeal secretions as an indicator of recent infection. Secretion specimens submitted for pertussis culture were examined for PsIgA by this technique. Of 348 specimens tested, B. pertussis was cultured from 57, and PsIgA was detected in 8 culture-positive and 40 culture-negative specimens. The average time between onset of symptoms and specimen collection for the culture-positive, PsIgA-negative specimens was 10 days; for the culture-positive, PsIgA-positive specimens, 15 days; and for the culture-negative, PsIgA-positive specimens, 36 days. Examination of paired samples available from several culture-proven cases demonstrated conversion from a negative PsIgA in the early sample to a positive PsIgA in the follow-up sample. Our results indicate that PsIgA is produced during natural human infection and does not arise as a result of parenteral vaccination. PsIgA usually appears in the nasopharyngeal secretions during the second or third week of illness and persists for at least 3 months. The detection of PsIgA in secretions may be a valuable diagnostic aid in culture-negative patients with pertussis.
开发了一种酶联免疫吸附测定法，用于检测鼻咽分泌物中针对百日咳博德特氏菌的免疫球蛋白A（IgA）抗体（PsIgA），作为近期感染的指标。采用该技术对提交进行百日咳培养的分泌物标本检测PsIgA。在检测的348份标本中，57份培养出百日咳博德特氏菌，8份培养阳性标本和40份培养阴性标本中检测到PsIgA。培养阳性、PsIgA阴性标本从症状出现到标本采集的平均时间为10天；培养阳性、PsIgA阳性标本为15天；培养阴性、PsIgA阳性标本为36天。对几例培养确诊病例的配对样本检测显示，早期样本中PsIgA为阴性，随访样本中转为阳性。我们的结果表明，PsIgA是在人类自然感染期间产生的，并非因注射疫苗所致。PsIgA通常在发病后第二或第三周出现在鼻咽分泌物中，并持续至少3个月。在分泌物中检测PsIgA对培养阴性的百日咳患者可能是一种有价值的诊断辅助手段。
