DNA紫外线损伤切除修复过程中染色质的瞬时构象变化。
Transient conformation changes in chromatin during excision repair of ultraviolet damage to DNA.
作者信息
Bodell W J, Cleaver J E
出版信息
Nucleic Acids Res. 1981 Jan 10;9(1):203-13. doi: 10.1093/nar/9.1.203.
Abstract
DNA labeled for 15 minutes during UV induced repair synthesis is two-fold more sensitive to micrococcal nuclease than the bulk nuclear DNA. As the length of the labeling period increases from 15 minutes to 4 hours the nuclease sensitivity of repair labeled DNA approaches that of bulk chromatin. Pulse-chase experiments indicate that the nuclease sensitivity of the repaired DNA labeled during a brief pulse decreases with a half-life of about 15 minutes. In contrast to previous interpretations, we consider these results to mean that immediately after synthesis, chromatin labeled during repair has a conformation which renders it more susceptible to nuclease digestion than the bulk chromatin. With time these repaired regions are assembled into a nucleosome structure with normal nuclease sensitivity.
摘要
在紫外线诱导的修复合成过程中标记15分钟的DNA，对微球菌核酸酶的敏感性是整体核DNA的两倍。随着标记时间从15分钟增加到4小时，修复标记DNA的核酸酶敏感性接近整体染色质的敏感性。脉冲追踪实验表明，在短暂脉冲期间标记的修复DNA的核酸酶敏感性以约15分钟的半衰期下降。与先前的解释相反，我们认为这些结果意味着在合成后立即，修复过程中标记的染色质具有一种构象，使其比整体染色质更容易受到核酸酶消化。随着时间的推移，这些修复区域被组装成具有正常核酸酶敏感性的核小体结构。
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