鸡成红细胞中复制型和非复制型染色质的结构特征。
Features of the structure of replicating and non-replicating chromatin in chicken erythroblasts.
作者信息
Hewish D
出版信息
Nucleic Acids Res. 1977 Jun;4(6):1881-90. doi: 10.1093/nar/4.6.1881.
Abstract
The digestion by DNAase I of DNA synthesised by isolated chicken erythroblasts was examined in isolated nuclei. It was found that newly synthesised DNA was susceptible to DNAase I but matured to a relatively resistant form with increasing time after replication as observed in mammalian systems. The presence of trypsin in the digestion exposed all of the DNA to DNAase I action. Examination of the digestion products showed that the newly replicated DNA differed little from the more mature form in the structure of the DNA-protein complex but that the difference in susceptibility was probably a result of a differential rate of access of the DNAase to the new and old DNA.
摘要
在分离的细胞核中检测了DNA酶I对分离的鸡红细胞合成的DNA的消化作用。结果发现，新合成的DNA对DNA酶I敏感，但随着复制后时间的增加，会成熟为相对抗性的形式，这与在哺乳动物系统中观察到的情况一致。消化过程中胰蛋白酶的存在使所有DNA都暴露于DNA酶I的作用之下。对消化产物的检测表明，新复制的DNA在DNA-蛋白质复合物的结构上与更成熟的形式差异不大，但敏感性的差异可能是由于DNA酶接触新旧DNA的速率不同所致。
相似文献
1
Features of the structure of replicating and non-replicating chromatin in chicken erythroblasts.鸡成红细胞中复制型和非复制型染色质的结构特征。
Nucleic Acids Res. 1977 Jun;4(6):1881-90. doi: 10.1093/nar/4.6.1881.
2
Chromatin structure: a property of the higher structures of chromatin and in the time course of its formation during chromatin replication.染色质结构：染色质高级结构的一种特性以及在染色质复制过程中其形成的时间进程。
Nucleic Acids Res. 1976 Dec;3(12):3293-304. doi: 10.1093/nar/3.12.3293.
3
4
A novel magnesium-dependent structural transition of chicken erythrocyte chromatin in situ.鸡红细胞染色质原位的一种新型镁依赖性结构转变。
Biochim Biophys Acta. 1985 Mar 20;824(3):243-6. doi: 10.1016/0167-4781(85)90054-5.
5
Release of a globin gene enriched chromatin fraction from chicken erythrocyte nuclei following DNase II digestion.经脱氧核糖核酸酶II消化后，从鸡红细胞核中释放出富含珠蛋白基因的染色质组分。
Nucleic Acids Res. 1981 Dec 11;9(23):6471-85. doi: 10.1093/nar/9.23.6471.
6
Changes in chromatin structure at the replication fork. II The DNPs containing nascent DNA and a transient chromatin modification detected by DNAase I.复制叉处染色质结构的变化。II. 含有新生DNA的DNP以及DNA酶I检测到的瞬时染色质修饰
Nucleic Acids Res. 1981 Aug 25;9(16):3991-4005. doi: 10.1093/nar/9.16.3991.
7
Chromatin assembly in isolated mammalian nuclei.分离的哺乳动物细胞核中的染色质组装
Nucleic Acids Res. 1978 Feb;5(2):349-62. doi: 10.1093/nar/5.2.349.
8
Avian erythrocyte chromatin degradation: the progressive exposure of the dinucleosomal repeat by bovine-pancreatic-DNAase-I-armed probes and free DNAase-I.鸟类红细胞染色质降解：用牛胰脱氧核糖核酸酶I武装的探针和游离的脱氧核糖核酸酶I对二核小体重复序列的逐步暴露。
Nucleic Acids Res. 1982 Jan 22;10(2):665-73. doi: 10.1093/nar/10.2.665.
9
Some effects of calcium and magnesium ions on the activity of bovine pancreatic deoxyribonuclease A.钙和镁离子对牛胰脱氧核糖核酸酶A活性的一些影响。
Biochim Biophys Acta. 1975 Jun 16;395(2):201-12. doi: 10.1016/0005-2787(75)90159-8.
10
Nucleosomes associated with newly replicated DNA have an altered conformation.与新复制的DNA相关的核小体具有改变的构象。
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2717-21. doi: 10.1073/pnas.75.6.2717.
引用本文的文献
1
Genome-scale identification of Caenorhabditis elegans regulatory elements by tiling-array mapping of DNase I hypersensitive sites.通过DNA酶I超敏感位点的芯片平铺图谱对秀丽隐杆线虫调控元件进行全基因组规模的鉴定。
BMC Genomics. 2009 Feb 25;10:92. doi: 10.1186/1471-2164-10-92.
2
Genome-wide mapping of DNase hypersensitive sites using massively parallel signature sequencing (MPSS).使用大规模平行签名测序（MPSS）对DNA酶超敏位点进行全基因组图谱绘制。
Genome Res. 2006 Jan;16(1):123-31. doi: 10.1101/gr.4074106. Epub 2005 Dec 12.
3
Transient conformation changes in chromatin during excision repair of ultraviolet damage to DNA.DNA紫外线损伤切除修复过程中染色质的瞬时构象变化。
Nucleic Acids Res. 1981 Jan 10;9(1):203-13. doi: 10.1093/nar/9.1.203.
4
Multistep pathway for replication-dependent nucleosome assembly.依赖复制的核小体组装的多步骤途径。
Proc Natl Acad Sci U S A. 1989 Sep;86(17):6459-63. doi: 10.1073/pnas.86.17.6459.
5
Cations and the accessibility of chromatin to nucleases.阳离子与染色质对核酸酶的可及性
Nucleic Acids Res. 1979 Jun 25;6(8):2929-45. doi: 10.1093/nar/6.8.2929.
6
The influence of chromatin structure on the distribution of DNA repair synthesis studied by nuclease digestion.通过核酸酶消化研究染色质结构对DNA修复合成分布的影响。
Nucleic Acids Res. 1979 Jan;6(1):359-70. doi: 10.1093/nar/6.1.359.
7
Chromatin replication revealed by studies of animal cells and papovaviruses (simian virus 40 and polyoma virus).通过对动物细胞和乳头瘤病毒（猴病毒40和多瘤病毒）的研究揭示的染色质复制。
Microbiol Rev. 1979 Sep;43(3):297-319. doi: 10.1128/mr.43.3.297-319.1979.
本文引用的文献
1
Crystalline desoxyribonuclease; isolation and general properties; spectrophotometric method for the measurement of desoxyribonuclease activity.结晶脱氧核糖核酸酶；分离及一般性质；测定脱氧核糖核酸酶活性的分光光度法
J Gen Physiol. 1950 Mar;33(4):349-62. doi: 10.1085/jgp.33.4.349.
2
A comparison of histones from chicken tissues by zone electrophoresis in starch gel.通过淀粉凝胶区带电泳对鸡组织中的组蛋白进行比较。
Can J Biochem Physiol. 1961 Mar;39:485-91. doi: 10.1139/o61-046.
3
Internal structure of the chromatin subunit.染色质亚基的内部结构。
Nucleic Acids Res. 1974 Nov;1(11):1573-8. doi: 10.1093/nar/1.11.1573.
4
Calcium-dependent priming of DNA synthesis in isolated rat liver nuclei.离体大鼠肝细胞核中DNA合成的钙依赖性引发
Biochem Biophys Res Commun. 1970 Apr 24;39(2):254-9. doi: 10.1016/0006-291x(70)90786-2.
5
DNA synthesis in purified populations of avian erythroid cells.禽类红细胞纯化群体中的DNA合成。
J Cell Sci. 1972 Jan;10(1):27-46. doi: 10.1242/jcs.10.1.27.
6
Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.染色质亚结构。一种核脱氧核糖核酸酶对染色质DNA在规则间隔位点的消化作用。
Biochem Biophys Res Commun. 1973 May 15;52(2):504-10. doi: 10.1016/0006-291x(73)90740-7.
7
Synthesis and turnover of DNA-bound histone during maturation of avian red blood cells.鸟类红细胞成熟过程中与DNA结合的组蛋白的合成与周转
J Mol Biol. 1972 Oct 14;70(3):425-34. doi: 10.1016/0022-2836(72)90550-5.
8
The calcium dependent endonuclease activity of isolated nuclear preparations. Relationships between its occurrence and the occurrence of other classes of enzymes found in nuclear preparations.分离的细胞核制剂的钙依赖性核酸内切酶活性。其出现与细胞核制剂中发现的其他种类酶的出现之间的关系。
Biochem Biophys Res Commun. 1973 May 15;52(2):475-81. doi: 10.1016/0006-291x(73)90736-5.
9
Mammalian chromatin substructure studies with the calcium-magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis.利用钙镁内切核酸酶和二维聚丙烯酰胺凝胶电泳进行的哺乳动物染色质亚结构研究。
Biochem J. 1974 Oct;143(1):67-72. doi: 10.1042/bj1430067.
10
Chromatographic separation of chromatin subunits.染色质亚基的色谱分离
Biochem Biophys Res Commun. 1974 Dec 23;61(4):1193-8. doi: 10.1016/s0006-291x(74)80410-9.
Zotero 插件