Müller W, Hattesohl I, Schuetz H J, Meyer G
Nucleic Acids Res. 1981 Jan 10;9(1):95-119. doi: 10.1093/nar/9.1.95.
Various base pair specific DNA ligands comprising a phenyl phenazinium dye, a triphenylmethan dye and Hoechst 33258 were covalently bound to polyethylene glycol (PEG) via ester or ether bonds. The DNA interactions of the PEG derivatives formed were shown to exhibit the same base pair specificity as the parent compounds. Since the PEG chains thus bound to the DNA could be expected to increase drastically the frictional coefficient of the DNA, the PEG derivatives were used for base specific DNA separations in agarose and polyacrylamide gel electrophoresis. The procedures, which do not require any special techniques, are described in detail. The resolution observed in agarose gels allows one to separate equally sized DNA fragments differing as little as 1% in base composition at mean travel distances of about 10 cm. Examples of gels showing the base compositional heterogeneity of restriction fragments obtained from lambda DNA, E. coli DNA and calf thymus DNA are given.
各种包含苯基吩嗪鎓染料、三苯甲烷染料和Hoechst 33258的碱基对特异性DNA配体通过酯键或醚键与聚乙二醇（PEG）共价结合。所形成的PEG衍生物与DNA的相互作用表现出与母体化合物相同的碱基对特异性。由于这样与DNA结合的PEG链有望大幅增加DNA的摩擦系数，因此PEG衍生物被用于琼脂糖和聚丙烯酰胺凝胶电泳中的碱基特异性DNA分离。详细描述了这些不需要任何特殊技术的方法。在琼脂糖凝胶中观察到的分辨率使人们能够在平均迁移距离约10厘米的情况下，分离出碱基组成差异仅为1%的等大小DNA片段。给出了显示从λDNA、大肠杆菌DNA和小牛胸腺DNA获得的限制性片段碱基组成异质性的凝胶示例。
