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一种鉴定限制性内切核酸酶识别的回文序列的简单方法：AvaII位点的核苷酸序列。

A simple method for identifying the palindromic sequences recognized by restriction endonucleases: the nucleotide sequence of the AvaII site.

作者信息

Fuchs C, Rosenvold E C, Honigman A, Szybalski W

出版信息

Gene. 1978 Sep;4(1):1-23. doi: 10.1016/0378-1119(78)90011-2.

DOI:10.1016/0378-1119(78)90011-2
PMID:215495
Abstract

Tables specifying the frequencies, distances between and positions of all possible tetra-, penta- and hexanucleotide palindromes in phiX174 and SV40 viral DNAs were prepared by a computer search of their base sequences. A simple method based on these tables is described for identifying the sequence recognized by any specific restriction endonuclease. The method requires experimental determination of the number and approximate sizes of the fragments obtained by digestion of phiX174 RF and SV40 DNAs. Using this method we identified the sequence for AvaII restriction endonuclease as 5'-GG(AT)CC.

摘要

通过对噬菌体φX174和猴空泡病毒40（SV40）的碱基序列进行计算机搜索，制备了指定这两种病毒DNA中所有可能的四核苷酸、五核苷酸和六核苷酸回文序列的频率、间距及位置的表格。本文描述了一种基于这些表格的简单方法，用于鉴定任何特定限制性内切酶所识别的序列。该方法需要通过实验确定噬菌体φX174复制型（RF）和SV40 DNA消化后得到的片段数量及大致大小。利用此方法，我们确定了AvaII限制性内切酶的识别序列为5'-GG(AT)CC。

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