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Characterization of conformational changes in (Na,K) ATPase labeled with fluorescein at the active site.

作者信息

Karlish S J

出版信息

J Bioenerg Biomembr. 1980 Aug;12(3-4):111-36. doi: 10.1007/BF00744678.

DOI:10.1007/BF00744678
PMID:6260765
Abstract
摘要

相似文献

1
Characterization of conformational changes in (Na,K) ATPase labeled with fluorescein at the active site.对在活性位点用荧光素标记的（钠，钾）-ATP酶构象变化的表征。
J Bioenerg Biomembr. 1980 Aug;12(3-4):111-36. doi: 10.1007/BF00744678.
2
Determination of monoclonal antibody-induced alterations in Na+/K+-ATPase conformations using fluorescein-labeled enzyme.使用荧光素标记的酶测定单克隆抗体诱导的Na+/K+-ATP酶构象变化。
Biochim Biophys Acta. 1989 Mar 16;995(1):42-53. doi: 10.1016/0167-4838(89)90231-8.
3
Characterization of ouabain-sensitive phosphatase activity in the absence of potassium ion in purified pig kidney Na,K-ATPase.纯化猪肾钠钾ATP酶中在无钾离子情况下哇巴因敏感性磷酸酶活性的表征
J Biochem. 1986 Jun;99(6):1613-24. doi: 10.1093/oxfordjournals.jbchem.a135635.
4
Use of formycin nucleotides, intrinsic protein fluorescence, and fluorescein isothiocyanate-labeled enzymes for measurement of conformational states of Na+,K+-ATPase.
Methods Enzymol. 1988;156:271-7. doi: 10.1016/0076-6879(88)56027-5.
5
Conformational transitions in fluorescein-labeled (Na,K)ATPase reconstituted into phospholipid vesicles.重组到磷脂囊泡中的荧光素标记的（钠，钾）ATP酶的构象转变
J Biol Chem. 1986 May 15;261(14):6248-54.
6
Estimation of the distance change between cysteine-457 and the nucleotide binding site when sodium pump changes conformation from E1 to E2 by fluorescence energy transfer measurements.通过荧光能量转移测量法估算钠泵从E1构象转变为E2构象时半胱氨酸-457与核苷酸结合位点之间的距离变化。
Biochemistry. 1996 Jun 25;35(25):8419-28. doi: 10.1021/bi960407+.
7
Dynamic lipid-protein stoichiometry on E1 and E2 conformations of the Na+/K+ -ATPase.钠钾 ATP 酶 E1 和 E2 构象上的动态脂质-蛋白计量比。
FEBS Lett. 2011 Apr 20;585(8):1153-7. doi: 10.1016/j.febslet.2011.03.024. Epub 2011 Mar 21.
8
Identification and localization of the 5-iodoacetamido-fluorescein reporter site on dog kidney Na,K-ATPase.
Prog Clin Biol Res. 1988;273:39-44.
9
Interaction of melittin with the (Na+ + K+)ATPase: evidence for a melittin-induced conformational change.
Arch Biochem Biophys. 1990 Dec;283(2):249-57. doi: 10.1016/0003-9861(90)90639-g.
10
A monoclonal antibody against a native conformation of the porcine renal Na+/K(+)-ATPase alpha-subunit protein.一种针对猪肾Na+/K(+)-ATP酶α亚基蛋白天然构象的单克隆抗体。
Biochim Biophys Acta. 1990 Sep 3;1040(2):267-75. doi: 10.1016/0167-4838(90)90086-u.

引用本文的文献

1
The effect of cytochrome c on Na,K-ATPase.细胞色素 c 对 Na,K-ATP 酶的影响。
J Bioenerg Biomembr. 2024 Jun;56(3):221-234. doi: 10.1007/s10863-024-10012-3. Epub 2024 Mar 22.
2
Molecular Mechanisms and Kinetic Effects of FXYD1 and Phosphomimetic Mutants on Purified Human Na,K-ATPase.FXYD1及拟磷酸化突变体对纯化的人钠钾ATP酶的分子机制和动力学效应
J Biol Chem. 2015 Nov 27;290(48):28746-59. doi: 10.1074/jbc.M115.687913. Epub 2015 Oct 1.
3
Stimulation, inhibition, or stabilization of Na,K-ATPase caused by specific lipid interactions at distinct sites.

本文引用的文献

1
Biochemical aspects of active transport.主动运输的生物化学方面
Annu Rev Biochem. 1967;36:727-56. doi: 10.1146/annurev.bi.36.070167.003455.
2
ATP hydrolysis associated with an uncoupled sodium flux through the sodium pump: evidence for allosteric effects of intracellular ATP and extracellular sodium.与通过钠泵的非偶联钠通量相关的ATP水解：细胞内ATP和细胞外钠变构效应的证据。
J Physiol. 1976 Apr;256(2):465-96. doi: 10.1113/jphysiol.1976.sp011333.
3
Defective conformational response in a selectively trypsinized (Na+ + K+)-ATPase studied with tryptophan fluorescence.
由不同位点的特定脂质相互作用引起的钠钾ATP酶的刺激、抑制或稳定作用。
J Biol Chem. 2015 Feb 20;290(8):4829-4842. doi: 10.1074/jbc.M114.611384. Epub 2014 Dec 22.
4
Extracellular allosteric Na(+) binding to the Na(+),K(+)-ATPase in cardiac myocytes.心肌细胞中细胞外别构 Na(+) 与 Na(+)，K(+)-ATP 酶的结合。
Biophys J. 2013 Dec 17;105(12):2695-705. doi: 10.1016/j.bpj.2013.11.004.
5
Dual mechanisms of allosteric acceleration of the Na(+),K(+)-ATPase by ATP.ATP 对 Na(+),K(+)-ATPase 变构加速的双重机制。
Biophys J. 2010 May 19;98(10):2290-8. doi: 10.1016/j.bpj.2010.01.038.
6
Neutralization of the charge on Asp 369 of Na+,K+-ATPase triggers E1 <--> E2 conformational changes.钠钾ATP酶天冬氨酸369位点电荷的中和引发E1⇌E2构象变化。
J Biol Chem. 2009 Nov 6;284(45):31038-51. doi: 10.1074/jbc.M109.050054. Epub 2009 Sep 2.
7
Structural changes in the catalytic cycle of the Na+,K+-ATPase studied by infrared spectroscopy.通过红外光谱研究钠钾ATP酶催化循环中的结构变化。
Biophys J. 2009 Apr 22;96(8):3433-42. doi: 10.1016/j.bpj.2009.01.010.
8
Solubilization, purification, and reconstitution of alpha 2 beta 1 isozyme of Na+/K+ -ATPase from caveolae of pulmonary smooth muscle plasma membrane: comparative studies with DHPC, C12E8, and Triton X-100.肺平滑肌质膜小窝中Na+/K+-ATP酶α2β1同工酶的增溶、纯化及重组：与二己酰磷脂酰胆碱（DHPC）、十二烷基聚氧乙烯醚（C12E8）和 Triton X-100的比较研究
Mol Cell Biochem. 2009 Mar;323(1-2):169-84. doi: 10.1007/s11010-008-9977-0. Epub 2008 Dec 20.
9
Divalent cation interactions with Na,K-ATPase cytoplasmic cation sites: implications for the para-nitrophenyl phosphatase reaction mechanism.二价阳离子与钠钾ATP酶胞质阳离子位点的相互作用：对对硝基苯磷酸酶反应机制的影响。
J Membr Biol. 2007 Mar;216(1):49-59. doi: 10.1007/s00232-007-9028-x. Epub 2007 Jun 17.
10
Kinetic characterization of Na,K-ATPase inhibition by Eosin.曙红对钠钾ATP酶抑制作用的动力学特征
Blood Cells Mol Dis. 2007 May-Jun;38(3):229-37. doi: 10.1016/j.bcmd.2007.01.001. Epub 2007 Feb 28.
用色氨酸荧光研究选择性胰蛋白酶处理的（钠+钾）-ATP酶中的缺陷构象反应。
Biochim Biophys Acta. 1980 Apr 10;597(2):305-17. doi: 10.1016/0005-2736(80)90108-x.
4
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
5
The interaction of sodium and potassium with the sodium pump in red cells.钠和钾与红细胞中钠泵的相互作用。
J Physiol. 1973 Jun;231(2):297-325. doi: 10.1113/jphysiol.1973.sp010234.
6
Kinetic properties of phosphorylated intermediates in the reaction of Na+, K+-ATPase.
Ann N Y Acad Sci. 1974;242(0):92-105. doi: 10.1111/j.1749-6632.1974.tb19082.x.
7
Purification and characterization of (Na+ plus K+ )-ATPase. IV. Estimation of the purity and of the molecular weight and polypeptide content per enzyme unit in preparations from the outer medulla of rabbit kidney.（钠钾）-ATP酶的纯化与特性研究。IV. 兔肾外髓质制剂中每酶单位的纯度、分子量及多肽含量的测定
Biochim Biophys Acta. 1974 Jul 12;356(1):53-67. doi: 10.1016/0005-2736(74)90293-4.
8
Purification and characterization of (Na+ plus K+ )-ATPase. 3. Purification from the outer medulla of mammalian kidney after selective removal of membrane components by sodium dodecylsulphate.（钠钾）-ATP酶的纯化与特性研究。3. 用十二烷基硫酸钠选择性去除膜成分后从哺乳动物肾脏外髓质中纯化该酶 。
Biochim Biophys Acta. 1974 Jul 12;356(1):36-52. doi: 10.1016/0005-2736(74)90292-2.
9
Photoaffinity labeling of the ouabain-binding site on (Na+ plus K+) adenosinetriphosphatase.（钠钾）三磷酸腺苷酶上哇巴因结合位点的光亲和标记
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2352-6. doi: 10.1073/pnas.71.6.2352.
10
Flip-flop model of (NaK)-ATPase function.（钠钾）-ATP酶功能的翻转模型
Acta Biol Med Ger. 1973;31(4):Suppl:K19-30.