Donehower L A, Huang A L, Hager G L
J Virol. 1981 Jan;37(1):226-38. doi: 10.1128/JVI.37.1.226-238.1981.
Molecular clones containing the 3' half of newly integrated mouse mammary tumor virus (MMTV) DNA with adjacent mouse cellular sequences were characterized. In addition, we cloned the long terminal redundancy joint from the unintegrated circular form of MMTV DNA. The entire nucleotide sequence of the integrated and part of the unintegrated terminal redundancy was determined; this allowed us to delineate the boundaries of the MMTV long terminal redundancy, which comprises 1,327 base pairs. The position of possible RNA polymerase II initiation and termination signals corresponded closely to the expected regions of viral RNA initiation and termination specified by current models. The MMTV long terminal redundancy also contained a large open reading frame with sufficient information for a protein of 198 amino acids. Initial comparison of flanking 3' cellular sequences from three independent integrated clones suggested there was no host sequence specificity in the MMTV integration event. However, specificity of integration with respect to viral sequences was precise.
对含有新整合的小鼠乳腺肿瘤病毒（MMTV）DNA 3' 端一半以及相邻小鼠细胞序列的分子克隆进行了表征。此外，我们从MMTV DNA的未整合环状形式中克隆了长末端冗余接头。确定了整合的以及部分未整合末端冗余的完整核苷酸序列；这使我们能够描绘出MMTV长末端冗余的边界，其由1327个碱基对组成。可能的RNA聚合酶II起始和终止信号的位置与当前模型指定的病毒RNA起始和终止的预期区域密切对应。MMTV长末端冗余还包含一个大的开放阅读框，具有足够的信息编码一个198个氨基酸的蛋白质。对来自三个独立整合克隆的侧翼3' 细胞序列的初步比较表明，MMTV整合事件中不存在宿主序列特异性。然而，就病毒序列而言，整合的特异性是精确的。
