DNA酶II消化后染色质颗粒中的蛋白质分布。

Protein distribution amongst chromatin particles following DNAse II digestion.

作者信息

Billett M A

出版信息

Cell Biol Int Rep. 1981 Jan;5(1):55-8. doi: 10.1016/0309-1651(81)90157-0.

PMID:6261968

Abstract

When active chromatin is released as a Mg-soluble fraction following digestion of nuclei with DNAse II, as concomitant release of HMG proteins, and hnRNP particles occurs. Release of HMG 14 and 17 is dependent on active chromatin release, whereas HMG 1 and 2, and hnRNP particles are released in an independent process. The Mg-soluble fraction comprises a heterogenous mixture of particles of less compact conformation than normal nucleosomes, and prone to protein-induced aggregation. Histone H1, and HMG 14 and 17 appear to be associated with these particles in a reversible manner, whereas HMG 1 and 2 are unbound.

摘要

当用DNA酶II消化细胞核后，活性染色质作为一种可溶于镁的组分被释放出来时，会伴随HMG蛋白和核不均一核糖核蛋白颗粒的释放。HMG 14和17的释放依赖于活性染色质的释放，而HMG 1和2以及核不均一核糖核蛋白颗粒则在一个独立的过程中被释放。可溶于镁的组分包含一种颗粒的异质混合物，其构象不如正常核小体紧密，并且易于发生蛋白质诱导的聚集。组蛋白H1以及HMG 14和17似乎以可逆的方式与这些颗粒相关联，而HMG 1和2则未结合。