Lutter L C
Nucleic Acids Res. 1981 Sep 11;9(17):4251-65. doi: 10.1093/nar/9.17.4251.
The precise locations and relative exposures of the DNase II-accessible sites in the nucleosome core DNA are determined using techniques previously employed for the enzyme DNase I. It is found that there are a number of similarities between the site exposure patterns for the two enzymes but that in general the DNase II seems to discriminate less among adjacent sites' accessibilities than does DNase I. The two enzymes attack essentially the same positions in the DNA, the average difference between the precise location of the site being less than one-half base for the two enzymes. Such close similarities in the digestion patterns of two enzymes with such different mechanisms of scission show that the patterns reflect the structure of the nucleosome core and not merely the properties of the particular enzyme used.
利用先前用于脱氧核糖核酸酶I的技术，确定核小体核心DNA中脱氧核糖核酸酶II可及位点的精确位置和相对暴露情况。发现这两种酶的位点暴露模式存在一些相似之处，但一般来说，脱氧核糖核酸酶II在相邻位点可及性之间的区分似乎比脱氧核糖核酸酶I少。这两种酶在DNA上攻击的基本是相同的位置，两种酶位点精确位置的平均差异小于半个碱基。两种具有如此不同切割机制的酶在消化模式上如此紧密的相似性表明，这些模式反映的是核小体核心的结构，而不仅仅是所用特定酶的特性。
