Adams D M
Appl Microbiol. 1974 Apr;27(4):797-801. doi: 10.1128/am.27.4.797-801.1974.
The requirement of ultrahigh temperature (UHT)-treated Clostridium perfringens spores for lysozyme and the sensitivity of heated and unheated spores to lysozyme were studied. The UHT-treated spores requiring lysozyme for germination and colony formation originated from only a small portion of the non-UHT-treated spore population. This raised a question of whether the requirement for lysozyme was natural to the spores or was induced by the UHT treatments. However, these spores did not require lysozyme for germination before UHT treatment, which confirmed that the requirement for lysozyme had been induced by the UHT treatment. Only 1 to 2% of the spores were naturally sensitive to lysozyme; therefore, the mere addition of lysozyme to the plating medium did not permit the enumeration of all survivors. Treatment of UHT-treated spores with ethylenediaminetetraacetate (EDTA) sensitized the spores to lysozyme and increased by 10- to 100-fold the number of survivors that were detected on a medium containing lysozyme. Under the heating conditions used, spores that were naturally sensitive to lysozyme and spores that required EDTA treatment were equally heat resistant.
研究了超高温（UHT）处理的产气荚膜梭菌孢子对溶菌酶的需求以及加热和未加热孢子对溶菌酶的敏感性。需要溶菌酶才能发芽和形成菌落的UHT处理孢子仅来源于一小部分未进行UHT处理的孢子群体。这就提出了一个问题，即对溶菌酶的需求是孢子固有的，还是由UHT处理诱导产生的。然而，这些孢子在UHT处理之前发芽并不需要溶菌酶，这证实了对溶菌酶的需求是由UHT处理诱导产生的。只有1%至2%的孢子对溶菌酶天然敏感；因此，仅在平板培养基中添加溶菌酶并不能对所有存活菌进行计数。用乙二胺四乙酸（EDTA）处理UHT处理过的孢子会使孢子对溶菌酶敏感，并使在含有溶菌酶的培养基上检测到的存活菌数量增加10至100倍。在所使用的加热条件下，对溶菌酶天然敏感的孢子和需要EDTA处理的孢子具有同等的耐热性。
