Raab-Traub N, Pritchett R, Kieff E
J Virol. 1978 Aug;27(2):388-98. doi: 10.1128/JVI.27.2.388-398.1978.
Previous kinetic and absorption hybridization experiments had demonstrated that the DNA of the B95-8 strain of Epstein-Barr virus was missing approximately 10% of the DNA sequences present in the DNA of the HR-1 strain (R.F. Pritchett, S.D. Hayward, and E. Kieff, J. Virol. 15:556-569, 1975; B. Sugder, W.C. Summers, and G. Klein, J. Virol. 18:765-775, 1976). The HR-1 strain differs from other laboratory strains, including the B95-8 and W91 strains, and from virus present in throat washings from patients with infectious mononucleosis in its inability to transform lymphocytes into lymphoblasts capable of long-term growth in culture (P. Gerber, Lancet i:1001, 1973; J. Menezes, W. Leibold, and G. Klein, Exp. Cell. Res. 92:478-484, 1975; G. Miller, D. Coope, J. Niederman, and J. Pagano, J. Virol. 18:1071-1080, 1976; G. Miller, J. Robinson, L. Heston, and M. Lipman, Proc. Natl. Acad. Sci. U.S.A. 71:4006-4010, 1974). In the experiments reported here, the restriction enzyme fragments of Epstein-Barr virus DNA which contain sequences which differ among the HR-1, B95-8, and W91 strains have been identified. The DNA of the HR-1, B95-8, and W91 strains each differed in complexity. The sequences previously shown to be missing in the B95-8 strain were contained in the EcoRI-C and -D and Hsu I-E and -N fragments of the HR-1 strain and in the EcoRI-C and Hsu I-D and -E fragments of the W91 strain. The HR-1 strain was missing DNA contained in EcoRI fragments A and J through K and Hsu I fragment B of the B95-8 strain and in the EcoRI-A and Hsu I-B fragments of the W91 strain. The relationship of these data to the linkage map of restriction enzyme fragments of the DNA of the B95-8 and W91 strains (E. Kieff, N. Raab-Traub, D. Given, W. King, A.T. Powell, R. Pritchett, and T. Dambaugh, In F. Rapp and G. de-The, ed., Oncogenesis and Herpesviruses III, in press; D. Given and E. Kieff, submitted for publication) and the possible significance of the data are discussed.
先前的动力学和吸收杂交实验表明，爱泼斯坦 - 巴尔病毒B95 - 8株的DNA缺失了HR - 1株DNA中约10%的DNA序列（R.F.普里切特、S.D.海沃德和E.基夫，《病毒学杂志》15:556 - 569，1975年；B.萨格德、W.C.萨默斯和G.克莱因，《病毒学杂志》18:765 - 775，1976年）。HR - 1株与其他实验室毒株不同，包括B95 - 8和W91株，也与传染性单核细胞增多症患者咽洗液中的病毒不同，在于它无法将淋巴细胞转化为能够在培养中长期生长的淋巴母细胞（P.格伯，《柳叶刀》i:1001，1973年；J.梅内塞斯、W.莱博尔德和G.克莱因，《细胞实验研究》92:478 - 484，1975年；G.米勒、D.库普、J.尼德曼和J.帕加诺，《病毒学杂志》18:1071 - 1080，1976年；G.米勒、J.罗宾逊、L.赫斯顿和M.利普曼，《美国国家科学院院刊》71:4006 - 4010，1974年）。在本文报道的实验中，已鉴定出爱泼斯坦 - 巴尔病毒DNA的限制性酶切片段，这些片段包含在HR - 1、B95 - 8和W91株之间不同的序列。HR - 1、B95 - 8和W91株的DNA在复杂性上各有不同。先前显示在B95 - 8株中缺失的序列包含在HR - 1株的EcoRI - C和 - D以及Hsu I - E和 - N片段中，以及W91株的EcoRI - C和Hsu I - D和 - E片段中。HR - 1株缺失了B95 - 8株EcoRI片段A和J至K以及Hsu I片段B中的DNA，以及W91株的EcoRI - A和Hsu I - B片段中的DNA。讨论了这些数据与B95 - 8和W91株DNA限制性酶切片段连锁图谱的关系（E.基夫、N.拉布 - 特劳布、D.吉文、W.金、A.T.鲍威尔.R.普里切特和T.丹博，载于F.拉普和G.德 - 泰编，《肿瘤发生与疱疹病毒III》，即将出版；D.吉文和E.基夫，已提交发表）以及这些数据可能的意义。
