Hsu T W, Taylor J M, Aldrich C, Townsend J B, Seal G, Mason W S
J Virol. 1981 Apr;38(1):219-23. doi: 10.1128/JVI.38.1.219-223.1981.
Previous study has shown that an avian sarcoma virus-transformed quail clone, Q-B77-11, apparently contains full-size proviral DNA and releases virus particles which contain polymerase activity, but are unable to form foci. Recently, we have found that after extended tissue culture of Q-B77-11, the predominant cell type changed from one in which there was a single copy of the integrated viral DNA to one with two copies in tandem. There was approximately one copy of the large terminal redundancy between these two tandem proviruses. Polyadenylic acid-containing RNA species of twice the size of the viral genome were detected in these cells and are interpreted as transcripts of the tandem provirus.
先前的研究表明，一种禽肉瘤病毒转化的鹌鹑克隆Q-B77-11显然含有全长前病毒DNA，并释放出含有聚合酶活性但无法形成病灶的病毒颗粒。最近，我们发现，在对Q-B77-11进行长期组织培养后，主要细胞类型从整合病毒DNA为单拷贝的细胞转变为串联有两个拷贝的细胞。在这两个串联前病毒之间大约有一个大末端冗余拷贝。在这些细胞中检测到了病毒基因组大小两倍的含聚腺苷酸的RNA种类，并被解释为串联前病毒的转录本。
