Samuel I, Tomas E, Bârnaure F
Virologie. 1981 Apr-Jun;32(2):145-54.
Influenza viruses A(H1N1) and A(H3N2) and parainfluenza viruses (Sendai mumps) were cultivated in chorioallantoic membrane (CAM) fragments maintained in media with different formulae, with or without daily medium changes, in roller or stationary tubes. Inoculation was performed either directly on CAM fragments in Petri dishes or by dilution of the virus-containing material in the medium. Infectant titers obtained in CAM fragments were similar to those recorded in embryonated eggs at 48 hours post inoculation (p.i.) in the case of influenza virus A(H1N1) and at 72 hours p.i. in that of Sendai and influenza A(H3N2) viruses; at 96 hours p.i. all the three viruses had titers superior to those found in the egg.
甲型流感病毒（H1N1）、甲型流感病毒（H3N2）和副流感病毒（仙台病毒）在含有不同配方培养基的绒毛尿囊膜（CAM）片段中培养，培养过程中培养基每日更换或不更换，培养容器为滚瓶或静止管。接种方式既可以直接接种在培养皿中的CAM片段上，也可以将含病毒材料在培养基中稀释后接种。对于甲型流感病毒（H1N1），接种后48小时，在CAM片段中获得的感染滴度与在鸡胚中记录的滴度相似；对于仙台病毒和甲型流感病毒（H3N2），接种后72小时，二者滴度相似；接种后96小时，三种病毒的滴度均高于在鸡胚中测得的滴度。
