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通过使用掺入磷脂囊泡中的纯化病毒和细胞抗原引发抗水泡性口炎病毒细胞毒性T淋巴细胞。

Elicitation of anti-vesicular stomatitis virus cytotoxic T lymphocytes by using purified viral and cellular antigens incorporated into phospholipid vesicles.

作者信息

Ruebush M J, Hale A H, Harris D T

出版信息

Infect Immun. 1981 May;32(2):513-7. doi: 10.1128/iai.32.2.513-517.1981.

DOI:10.1128/iai.32.2.513-517.1981
PMID:6265359
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC351476/
Abstract

We evaluated the minimal molecular and cellular requirements for elicitation of anti-vesicular stomatitis virus (VSV) cytotoxic T lymphocytes (CTL). The results indicated that lipid vesicles containing the purified major surface glyco-protein of VSV (G protein) and purified H-2K(k) glycoproteins elicited specific H-2K(k)-restricted anti-VSV CTL. These antiviral CTL were shown to be Ly 1(-),2(+). However, both Ly 1(+),2(-) and Ly1(-),2(+) T-cell subpopulations were shown to be required for elicitation of these CTL.

摘要

我们评估了引发抗水疱性口炎病毒(VSV)细胞毒性T淋巴细胞(CTL)所需的最小分子和细胞条件。结果表明,含有纯化的VSV主要表面糖蛋白(G蛋白)和纯化的H-2K(k)糖蛋白的脂质囊泡可引发特异性的H-2K(k)限制性抗VSV CTL。这些抗病毒CTL显示为Ly 1(-)、2(+)。然而,Ly 1(+)、2(-)和Ly1(-)、2(+) T细胞亚群均被证明是引发这些CTL所必需的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/828a/351476/0f810d6e6fde/iai00163-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/828a/351476/0f810d6e6fde/iai00163-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/828a/351476/0f810d6e6fde/iai00163-0112-a.jpg

相似文献

1
Elicitation of anti-vesicular stomatitis virus cytotoxic T lymphocytes by using purified viral and cellular antigens incorporated into phospholipid vesicles.通过使用掺入磷脂囊泡中的纯化病毒和细胞抗原引发抗水泡性口炎病毒细胞毒性T淋巴细胞。
Infect Immun. 1981 May;32(2):513-7. doi: 10.1128/iai.32.2.513-517.1981.
2
H-2L-restricted recognition of viral antigens In the H-2d haplotype, anti-vesicular stomatitis virus cytotoxic T cells are restricted solely by H-2L.H-2L对病毒抗原的限制性识别 在H-2d单倍型中,抗水泡性口炎病毒细胞毒性T细胞仅受H-2L的限制。
J Exp Med. 1982 Sep 1;156(3):778-90. doi: 10.1084/jem.156.3.778.
3
Possible mechanisms by which the H-2Kbm3 mutation may decrease cytotoxic T-lymphocyte recognition of vesicular stomatitis virus nucleoprotein antigen.H-2Kbm3突变可能降低细胞毒性T淋巴细胞对水疱性口炎病毒核蛋白抗原识别的潜在机制。
J Virol. 1987 Jun;61(6):1992-8. doi: 10.1128/JVI.61.6.1992-1998.1987.
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Synthetic phospholipid vesicles containing a purified viral antigen and cell membrane proteins stimulate the development of cytotoxic T lymphocytes.含有纯化病毒抗原和细胞膜蛋白的合成磷脂囊泡可刺激细胞毒性T淋巴细胞的发育。
J Exp Med. 1979 Nov 1;150(5):1067-74. doi: 10.1084/jem.150.5.1067.
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Vesicular stomatitis virus glycoprotein is necessary for H-2-restricted lysis of infected cells by cytotoxic T lymphocytes.水泡性口炎病毒糖蛋白对于细胞毒性T淋巴细胞对感染细胞进行H-2限制性裂解是必需的。
Proc Natl Acad Sci U S A. 1978 Feb;75(2):970-4. doi: 10.1073/pnas.75.2.970.
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Elicitation of anti-Sendai virus cytotoxic T lymphocytes by viral and H-2 antigens incorporated into the same lipid bilayer by membrane fusion and by reconstitution into liposomes.通过膜融合以及重组到脂质体中,将病毒和H-2抗原整合到同一脂质双层中,从而引发抗仙台病毒细胞毒性T淋巴细胞。
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Cytolytic T lymphocytes from the BALB/c-H-2dm2 mouse recognize the vesicular stomatitis virus glycoprotein and are restricted by class II MHC antigens.来自BALB/c-H-2dm2小鼠的细胞溶解性T淋巴细胞可识别水疱性口炎病毒糖蛋白,并受II类主要组织相容性复合体抗原的限制。
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Cross-reactive anti-vesicular stomatitis virus (VSV) cytotoxic T lymphocytes recognize the major surface glycoprotein.交叉反应性抗水泡性口炎病毒(VSV)细胞毒性T淋巴细胞识别主要表面糖蛋白。
Eur J Immunol. 1981 May;11(5):434-6. doi: 10.1002/eji.1830110517.
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The role of vesicular stomatitis virus major glycoprotein in determining the specificity of virus-specific and H-2-restricted cytolytic T cells.水疱性口炎病毒主要糖蛋白在决定病毒特异性和H-2限制性细胞毒性T细胞特异性中的作用。
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Lysis of target cells infected with vesicular stomatitis virus (VSV) in the presence of tunicamycin by anti-VSV cytotoxic T lymphocytes.在衣霉素存在的情况下,抗水疱性口炎病毒细胞毒性T淋巴细胞对感染水疱性口炎病毒(VSV)的靶细胞的裂解作用。
J Immunol. 1981 May;126(5):1914-8.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Inability of mice to generate cytotoxic T lymphocytes to vesicular stomatitis virus restricted to H-2Kk or H-2Dk.小鼠无法产生针对局限于H-2Kk或H-2Dk的水疱性口炎病毒的细胞毒性T淋巴细胞。
J Immunol. 1981 Feb;126(2):446-51.
3
Absence of interference during high-multiplicity infection by clonally purified vesicular stomatitis virus.克隆纯化的水疱性口炎病毒在高复数感染期间不存在干扰现象。
J Virol. 1971 Mar;7(3):409-11. doi: 10.1128/JVI.7.3.409-411.1971.
4
Ribonucleic acid synthesis of vesicular stomatitis virus. I. Species of ribonucleic acid found in Chinese hamster ovary cells infected with plaque-forming and defective particles.水疱性口炎病毒的核糖核酸合成。I. 在感染蚀斑形成颗粒和缺陷颗粒的中国仓鼠卵巢细胞中发现的核糖核酸种类。
J Virol. 1969 Aug;4(2):154-61. doi: 10.1128/JVI.4.2.154-161.1969.
5
The glycoprotein of vesicular stomatitis virus is the antigen that gives rise to and reacts with neutralizing antibody.水疱性口炎病毒的糖蛋白是产生中和抗体并与之发生反应的抗原。
J Virol. 1972 Dec;10(6):1231-5. doi: 10.1128/JVI.10.6.1231-1235.1972.
6
Purification of the H-2Kk molecule of the murine major histocompatibility complex.小鼠主要组织相容性复合体H-2Kk分子的纯化
J Biol Chem. 1979 Sep 25;254(18):8713-6.
7
Induction of secondary cytotoxic T lymphocytes by purified HLA-A and HLA-B antigens reconstituted into phospholipid vesicles.重组到磷脂囊泡中的纯化 HLA - A 和 HLA - B 抗原诱导继发性细胞毒性 T 淋巴细胞。
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5688-91. doi: 10.1073/pnas.75.11.5688.
8
Insertion of Ia and H-2 alloantigens into model membranes.
Proc Natl Acad Sci U S A. 1979 Feb;76(2):902-6. doi: 10.1073/pnas.76.2.902.
9
Synthetic phospholipid vesicles containing a purified viral antigen and cell membrane proteins stimulate the development of cytotoxic T lymphocytes.含有纯化病毒抗原和细胞膜蛋白的合成磷脂囊泡可刺激细胞毒性T淋巴细胞的发育。
J Exp Med. 1979 Nov 1;150(5):1067-74. doi: 10.1084/jem.150.5.1067.
10
Vesicular stomatitis virus glycoprotein is necessary for H-2-restricted lysis of infected cells by cytotoxic T lymphocytes.水泡性口炎病毒糖蛋白对于细胞毒性T淋巴细胞对感染细胞进行H-2限制性裂解是必需的。
Proc Natl Acad Sci U S A. 1978 Feb;75(2):970-4. doi: 10.1073/pnas.75.2.970.