Wright C D, Herron M J, Gray G R, Holmes B, Nelson R D
Infect Immun. 1981 May;32(2):731-8. doi: 10.1128/iai.32.2.731-738.1981.
We investigated the possibility that dissolved mannan polysaccharide from Candida may have an inhibitory influence on the host defense mechanisms associated with neutrophils and thereby contribute to the pathogenesis of candidoses. We created a model for this hypothesis by using mannan derived from bakers' yeast (Saccharomyces cerevisiae) and mannan isolated from the serum of a patient with chronic mucocutaneous candidosis. We observed that these mannans inhibited the respiratory burst and release of myeloperoxidase stimulated by phagocytosis of serum-opsonized zymosan in vitro. Mannan did not inhibit release of three other lysosomal enzymes. The selective inhibition of release of myeloperoxidase was attributable to a carbohydrate-binding property of the enzyme, with the mannan causing co-sedimentation of the enzyme with the cellular fraction.
我们研究了来自念珠菌的溶解性甘露聚糖多糖可能对与中性粒细胞相关的宿主防御机制产生抑制作用，从而导致念珠菌病发病的可能性。我们通过使用源自面包酵母（酿酒酵母）的甘露聚糖和从一名慢性黏膜皮肤念珠菌病患者血清中分离出的甘露聚糖，为这一假设建立了一个模型。我们观察到，这些甘露聚糖在体外抑制了血清调理酵母聚糖吞噬作用刺激的呼吸爆发和髓过氧化物酶的释放。甘露聚糖并不抑制其他三种溶酶体酶的释放。髓过氧化物酶释放的选择性抑制归因于该酶的碳水化合物结合特性，甘露聚糖导致该酶与细胞组分共沉降。
