Wright C D, Bowie J U, Nelson R D
Infect Immun. 1984 Feb;43(2):467-71. doi: 10.1128/iai.43.2.467-471.1984.
Structural features of mannan which participate in the formation of myeloperoxidase-mannan-neutrophil complexes have been studied by using a battery of structurally modified mannans. Mannan was isolated from Saccharomyces cerevisiae X2180 wild type and modified by strong alkaline degradation, selective (mild) alkaline degradation, and selective acetolysis. Mannose oligosaccharides bound to the peptide portion of mannan appeared to be required for binding of mannan to the neutrophil. The interaction of mannan with myeloperoxidase appeared to occur through phosphate groups of the mannan outer chain. The myeloperoxidase-mannan interaction was determined to be ionic in nature. The mannan-neutrophil interaction may involve cell membrane receptors for mannose.
通过使用一系列结构修饰的甘露聚糖，研究了参与髓过氧化物酶-甘露聚糖-中性粒细胞复合物形成的甘露聚糖的结构特征。甘露聚糖从酿酒酵母X2180野生型中分离出来，并通过强碱降解、选择性（温和）碱降解和选择性乙酰解进行修饰。与甘露聚糖肽部分结合的甘露糖寡糖似乎是甘露聚糖与中性粒细胞结合所必需的。甘露聚糖与髓过氧化物酶的相互作用似乎通过甘露聚糖外链的磷酸基团发生。髓过氧化物酶-甘露聚糖相互作用被确定为离子性质。甘露聚糖-中性粒细胞相互作用可能涉及甘露糖的细胞膜受体。
