Gliotoxin对人多形核中性粒细胞的影响。
Effect of gliotoxin on human polymorphonuclear neutrophils.
作者信息
Shah D T, Jackman S, Engle J, Larsen B
机构信息
Department of Pathology, Marshall University School of Medicine, Huntington, WV, USA.
出版信息
Infect Dis Obstet Gynecol. 1998;6(4):168-75. doi: 10.1002/(SICI)1098-0997(1998)6:4<168::AID-IDOG6>3.0.CO;2-Z.
OBJECTIVES
Candida albicans is known to produce gliotoxin, which has several prominent biological effects, including immunosuppression. Interference with host defenses may arise from the effects of this toxin on leukocyte structure and function.
METHODS
Flow cytometric analysis revealed that polymorphonuclear leukocytes (PMN) were more sensitive to gliotoxin than were mononuclear cells. Structural and various functional aspects of PMN exposed to gliotoxin were studied.
RESULTS
Gliotoxin at (1 microgram/mL) did not affect the viability but did diminish PMN chemotaxis and reduced their ability to ingest particles. Other functional aberrations included decreased nitroblue tetrazolium dye reduction, decreased superoxide production, and release of lactoferrin suggesting by degranulation. Gliotoxin also affected the ability of PMN to kill Escherichia coli.
CONCLUSIONS
This study suggests a previously unrecognized potential virulence factor of C. albicans that could contribute to persistence of yeast colonization or recurrence of symptomatic infection through diminished host resistance.
目的
已知白色念珠菌可产生胶质毒素，该毒素具有多种显著的生物学效应，包括免疫抑制。这种毒素对白细胞结构和功能的影响可能会干扰宿主防御。
方法
流式细胞术分析显示，多形核白细胞（PMN）对胶质毒素比单核细胞更敏感。研究了暴露于胶质毒素的PMN的结构和各种功能方面。
结果
1微克/毫升的胶质毒素不影响细胞活力，但会降低PMN的趋化性并降低其摄取颗粒的能力。其他功能异常包括硝基蓝四氮唑染料还原减少、超氧化物产生减少以及通过脱颗粒释放乳铁蛋白。胶质毒素还影响PMN杀死大肠杆菌的能力。
结论
本研究提示白色念珠菌存在一种先前未被认识的潜在毒力因子，它可能通过降低宿主抵抗力导致酵母菌定植持续存在或症状性感染复发。
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