Parker C, Romig W R
J Bacteriol. 1972 Nov;112(2):707-14. doi: 10.1128/jb.112.2.707-714.1972.
Vibrio cholerae cells, infected with the sex factor P, produce discrete, plaque-like clearings when plated on lawns of P(-) cells. We investigated the nature of these clearings and conclude that they are probably sites of active mating. We developed a quantitative assay for P(+) cells and used it to study the kinetics of sex factor spread in broth cultures. Both established and newly infected donor populations were efficient sex factor donors, indicating that P is not self-repressed. We also investigated the kinetics of recombinant formation in broth matings. In 1-hr matings, we routinely found recombination frequencies of 10(-6) per donor cell. Kinetic studies of recombinant formation showed that the markers tested all appeared at early times. Thus P, the V. cholerae sex factor, seems to resemble F in its transfer properties.
感染了性因子P的霍乱弧菌细胞，接种在P（-）细胞菌苔上时会产生离散的、菌斑样的清亮区域。我们研究了这些清亮区域的性质，并得出结论，它们可能是活跃交配的位点。我们开发了一种针对P（+）细胞的定量检测方法，并用于研究肉汤培养中性因子传播的动力学。已建立的和新感染的供体群体都是高效的性因子供体，这表明P不会自我抑制。我们还研究了肉汤交配中重组形成的动力学。在1小时的交配中，我们通常发现每个供体细胞的重组频率为10（-6）。重组形成的动力学研究表明，所测试的标记物在早期都出现了。因此，霍乱弧菌性因子P在其转移特性上似乎类似于F。
