黑腹果蝇的笨蛋突变体：在环磷酸腺苷磷酸二酯酶酶系统中存在缺陷的突变体。
Dunce mutants of Drosophila melanogaster: mutants defective in the cyclic AMP phosphodiesterase enzyme system.
作者信息
Davis R L, Kiger J A
出版信息
J Cell Biol. 1981 Jul;90(1):101-7. doi: 10.1083/jcb.90.1.101.
Abstract
The cyclic AMP and cyclic GMP phosphodiesterase activities present in flies of six mutant strains of the dunce gene and in the parent wild-type strains are characterized. All of the mutants exhibit aberrant cyclic AMP metabolism. The mutant strains dunceM14, dunceM11, and dunceML appear to be amorphic, because they completely lack the cAMP-specific phosphodiesterase normally present in adult flies. These strains exhibit extremely high levels of cAMP. The mutant strains dunce1, dunce2, and dunceCK are hypomorphic and exhibit reduced levels of the cAMP-specific phosphodiesterase. These strains exhibit less marked increases in cAMP content compared with the three amorphic strains. The dunce2 strain possesses a residual enzyme activity that exhibits anomalous kinetics compared with those of the normal enzyme. The possibility that the dunce locus is the structural gene for the cAMP-specific phosphodiesterase is discussed.
摘要
对六个“笨蛋”基因突变株系的果蝇以及亲本野生型株系中存在的环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）磷酸二酯酶活性进行了表征。所有突变体均表现出异常的cAMP代谢。“笨蛋”突变株系dunceM14、dunceM11和dunceML似乎是无效等位基因，因为它们完全缺乏成年果蝇中正常存在的cAMP特异性磷酸二酯酶。这些株系表现出极高水平的cAMP。“笨蛋”突变株系dunce1、dunce2和dunceCK是亚效等位基因，表现出cAMP特异性磷酸二酯酶水平降低。与三个无效等位基因株系相比，这些株系中cAMP含量的增加不太明显。dunce2株系具有残余酶活性，与正常酶相比，其动力学表现异常。文中讨论了“笨蛋”基因座是cAMP特异性磷酸二酯酶结构基因的可能性。
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