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用间接血凝试验检测滤纸上采集血液中巨细胞病毒抗体(作者译)

[Indirect hemagglutination test for detection of antibodies to cytomegalovirus in blood collected on blotting paper (author's transl)].

作者信息

Cabau N, Duros C, Ravisé N, Coulon M, Boué A

出版信息

Pathol Biol (Paris). 1976 Oct;24(8):575-9.

PMID:185575
Abstract

Indirect hemagglutination test for detection of antibodies to cytomegalovirus is highly sensitive and reproducible, if employed in well-defined conditions. Standardization of the various factors involved is necessary as well as their reciprocal equilibrium : sheep erythrocytes, antigen, dilution of tanin, buffers quality. The hemagglutination test can be performed on small volumes such as blood collection on blotting paper (PKU). Antibody titers were compared in the serum and the blood so collected in 104 subjects : the results were very similar and no "false negative" were found in any case. This way of collecting blood and hemagglutination are technical improvements in epidemiologic studies of cytomegalovirus infection. It can be hoped they will be adapted to other group herpes infections.

摘要

用于检测巨细胞病毒抗体的间接血凝试验,如果在明确界定的条件下使用,具有高度敏感性和可重复性。对所涉及的各种因素进行标准化以及它们之间的相互平衡是必要的,这些因素包括绵羊红细胞、抗原、单宁稀释度、缓冲液质量。血凝试验可以在小体积样本上进行,比如在吸水纸上采集的血液(苯丙酮尿症检测)。在104名受试者中比较了血清和如此采集的血液中的抗体滴度:结果非常相似,且在任何情况下均未发现“假阴性”。这种采血方式和血凝试验是巨细胞病毒感染流行病学研究中的技术改进。有望它们能适用于其他疱疹病毒感染组。

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