Weiss R, Birch-Hirschfeld E, Zimmer C, Reinert H, Luck G
Nucleic Acids Symp Ser. 1980(7):73-87.
Two types of deoxyoligonucleotides, a nona- and two dodecamers have been synthesized as a basis of various aspects in structural and functional studies of the DNA. Using different methodological approaches highly purified deoxyoligomers were obtained and characterized by UV absorption and CD measurements. As an example the results presented are concerned with the synthesis of the following dodecanucleotide containing the recognition site of Hind III : dC-A-C-A-A-G-C-T-T-G-T-G. CD properties and some structural related effects of two complementary and a selfcomplementary deoxyoligomers are described.
已合成了两种类型的脱氧寡核苷酸，一种九聚体和两种十二聚体，作为DNA结构和功能研究各个方面的基础。采用不同的方法获得了高纯度的脱氧寡聚物，并通过紫外吸收和圆二色性测量对其进行了表征。作为示例，所呈现的结果涉及包含Hind III识别位点的以下十二聚核苷酸的合成：dC-A-C-A-A-G-C-T-T-G-T-G。描述了两种互补和一种自互补脱氧寡聚物的圆二色性特性以及一些与结构相关的效应。
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