含7-脱氮腺嘌呤的寡脱氧核苷酸：合成及限制性内切酶识别
Oligodeoxynucleotides containing 7-deazaadenine: synthesis and recognition by restriction endonucleases.
作者信息
Ono A, Ohtani Y, Sato M, Ueda T
出版信息
Nucleic Acids Symp Ser. 1983(12):67-70.
PMID:6320131
Abstract
Deoxydecanucleotides containing a recognition sequence of Bg1 II and Sau 3AI, and their 7-deazaadenine analogs were synthesized by the phosphotriester method. The decanucleotides containing 7-deazaadenine in place of adenine were partially or strongly resistant to the hydrolysis by these restriction endonucleases.
摘要
通过磷酸三酯法合成了含有Bg1 II和Sau 3AI识别序列的脱氧十聚核苷酸及其7-脱氮腺嘌呤类似物。用7-脱氮腺嘌呤取代腺嘌呤的十聚核苷酸对这些限制性内切核酸酶的水解具有部分抗性或强抗性。
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