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含7-脱氮腺嘌呤的脱氧寡核苷酸的合成：限制性内切酶Bgl II和Sau 3AI的识别与切割（核苷与核苷酸 第55部分）

Synthesis of deoxyoligonucleotides containing 7-deazaadenine: recognition and cleavage by restriction endonuclease Bgl II and Sau 3AI (nucleosides and nucleotides Part 55).

作者信息

Ono A, Sato M, Ohtani Y, Ueda T

出版信息

Nucleic Acids Res. 1984 Dec 11;12(23):8939-49. doi: 10.1093/nar/12.23.8939.

DOI:10.1093/nar/12.23.8939
PMID:6096813
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320430/
Abstract

Deoxydecanucleotides having a recognition sequence of Bgl II and Sau 3AI, and their 7-deazaadenine analogs were synthesized. The decanucleotides containing 7-deazaadenine in place of adenine were partially resistant to the hydrolysis by Sau 3AI and strongly resistant to that by Bgl II. A new hypothesis on the mode of recognition and cleavage of specific nucleotide sequences by Bgl II, recognizing one strand and cleaving the other strand, is presented.

摘要

合成了具有Bgl II和Sau 3AI识别序列的脱氧十聚核苷酸及其7-脱氮腺嘌呤类似物。含有7-脱氮腺嘌呤取代腺嘌呤的十聚核苷酸对Sau 3AI的水解有部分抗性，对Bgl II的水解有强抗性。提出了一个关于Bgl II识别和切割特定核苷酸序列模式的新假说，即识别一条链并切割另一条链。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41ac/320430/4d79f89baca4/nar00341-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41ac/320430/4d79f89baca4/nar00341-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41ac/320430/4d79f89baca4/nar00341-0202-a.jpg

相似文献

1
Synthesis of deoxyoligonucleotides containing 7-deazaadenine: recognition and cleavage by restriction endonuclease Bgl II and Sau 3AI (nucleosides and nucleotides Part 55).含7-脱氮腺嘌呤的脱氧寡核苷酸的合成：限制性内切酶Bgl II和Sau 3AI的识别与切割（核苷与核苷酸 第55部分）
Nucleic Acids Res. 1984 Dec 11;12(23):8939-49. doi: 10.1093/nar/12.23.8939.
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引用本文的文献

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J Org Chem. 1998;63(26):9652-9656. doi: 10.1021/jo9805100.
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Nucleic Acids Res. 1985 Dec 20;13(24):8665-84. doi: 10.1093/nar/13.24.8665.
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本文引用的文献

1
DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.DNA序列分析：一种通过图谱对大的寡脱氧核糖核苷酸片段进行测序的通用、简单且快速的方法。
Nucleic Acids Res. 1974 Mar;1(3):331-53. doi: 10.1093/nar/1.3.331.
2
Solid-phase synthesis of polynucleotides. IV. Usage of polystyrene resins for the synthesis of polydeoxyribonucleotides by the phosphostriester method.多核苷酸的固相合成。IV. 磷酸三酯法中聚苯乙烯树脂在聚脱氧核糖核苷酸合成中的应用。
Nucleic Acids Res. 1980 Nov 25;8(22):5507-17. doi: 10.1093/nar/8.22.5507.
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Effect of nucleotide analogs on the cleavage of DNA by the restriction enzymes AluI, DdeI, HinfI, RsaI, and TaqI.
使用修饰的寡脱氧核苷酸分析EcoRV催化的DNA切割所涉及的识别机制。
Nucleic Acids Res. 1988 Dec 23;16(24):11781-93. doi: 10.1093/nar/16.24.11781.
4
Minor-groove-modified oligonucleotides: synthesis of decadeoxynucleotides containing hypoxanthine, N2-methylguanine and 3-deazaadenine, and their interactions with restriction endonucleases Bgl II, Sau, 3AI, and Mbo I (Nucleosides and Nucleotides Part 75).小沟修饰的寡核苷酸：含次黄嘌呤、N2-甲基鸟嘌呤和3-脱氮腺嘌呤的十聚脱氧核苷酸的合成及其与限制性内切酶Bgl II、Sau、3AI和Mbo I的相互作用（核苷与核苷酸第75部分）
Nucleic Acids Res. 1987 Apr 10;15(7):3059-72. doi: 10.1093/nar/15.7.3059.
5
Synthesis of decadeoxyribonucleotides containing N6-methyladenine, N4-methylcytosine, and 5-methylcytosine: recognition and cleavage by restriction endonucleases (nucleosides and nucleotides part 74).含N6-甲基腺嘌呤、N4-甲基胞嘧啶和5-甲基胞嘧啶的脱氧核糖核苷酸的合成：限制性内切酶的识别与切割（核苷与核苷酸第74部分）
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6
Alkyl phosphotriester modified oligodeoxyribonucleotides. V. Synthesis and absolute configuration of Rp and Sp diastereomers of an ethyl phosphotriester (Et) modified EcoRI recognition sequence, d[GGAA(Et)TTCC]. A synthetic approach to regio- and stereospecific ethylation-interference studies.烷基磷酸三酯修饰的寡脱氧核糖核苷酸。V. 磷酸三乙酯（Et）修饰的EcoRI识别序列d[GGAA(Et)TTCC]的Rp和Sp非对映异构体的合成及绝对构型。区域和立体特异性乙基干扰研究的合成方法。
Nucleic Acids Res. 1986 Sep 25;14(18):7405-20. doi: 10.1093/nar/14.18.7405.
7
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8
Restriction endonucleases HindII and TaqI cleave DNA with mismatched nucleotides within their recognition sequences.限制性内切核酸酶HindII和TaqI可在其识别序列内切割含有错配核苷酸的DNA。
Nucleic Acids Res. 1986 Mar 11;14(5):1943-9. doi: 10.1093/nar/14.5.1943.
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CD spectra and some properties of deoxyoligonucleotide duplexes having a C:G terminus (nucleosides and nucleotides. Part 69).具有C:G末端的脱氧寡核苷酸双链体的圆二色光谱及一些性质（核苷与核苷酸。第69部分）
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10
Interaction of the restriction endonuclease ScaI with its substrates.限制性内切酶ScaI与其底物的相互作用。
Nucleic Acids Res. 1985 Oct 11;13(19):7015-24. doi: 10.1093/nar/13.19.7015.
核苷酸类似物对限制性内切酶AluI、DdeI、HinfI、RsaI和TaqI切割DNA的影响。
J Biol Chem. 1983 Dec 25;258(24):15206-13.
4
Purification and properties of the Bg1I and II endonucleases.
Methods Enzymol. 1980;65(1):132-8. doi: 10.1016/s0076-6879(80)65018-6.
5
Sequence-specific recognition of double helical nucleic acids by proteins.蛋白质对双螺旋核酸的序列特异性识别。
Proc Natl Acad Sci U S A. 1976 Mar;73(3):804-8. doi: 10.1073/pnas.73.3.804.
6
A restriction endonuclease from Staphylococcus aureus.一种来自金黄色葡萄球菌的限制性内切酶。
Nucleic Acids Res. 1976 Nov;3(11):3193-202. doi: 10.1093/nar/3.11.3193.
7
Biochemical and genetic properties of site-specific restriction endonucleases in Bacillus globigii.
J Bacteriol. 1978 Apr;134(1):338-44. doi: 10.1128/jb.134.1.338-344.1978.
8
Structures and mechanisms of DNA restriction and modification enzymes.
Q Rev Biophys. 1979 Aug;12(3):315-69. doi: 10.1017/s0033583500005461.