含7-脱氮腺嘌呤的脱氧寡核苷酸的合成：限制性内切酶Bgl II和Sau 3AI的识别与切割（核苷与核苷酸 第55部分）
Synthesis of deoxyoligonucleotides containing 7-deazaadenine: recognition and cleavage by restriction endonuclease Bgl II and Sau 3AI (nucleosides and nucleotides Part 55).
作者信息
Ono A, Sato M, Ohtani Y, Ueda T
出版信息
Nucleic Acids Res. 1984 Dec 11;12(23):8939-49. doi: 10.1093/nar/12.23.8939.
Abstract
Deoxydecanucleotides having a recognition sequence of Bgl II and Sau 3AI, and their 7-deazaadenine analogs were synthesized. The decanucleotides containing 7-deazaadenine in place of adenine were partially resistant to the hydrolysis by Sau 3AI and strongly resistant to that by Bgl II. A new hypothesis on the mode of recognition and cleavage of specific nucleotide sequences by Bgl II, recognizing one strand and cleaving the other strand, is presented.
摘要
合成了具有Bgl II和Sau 3AI识别序列的脱氧十聚核苷酸及其7-脱氮腺嘌呤类似物。含有7-脱氮腺嘌呤取代腺嘌呤的十聚核苷酸对Sau 3AI的水解有部分抗性，对Bgl II的水解有强抗性。提出了一个关于Bgl II识别和切割特定核苷酸序列模式的新假说，即识别一条链并切割另一条链。
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