Salonen E M, Vaheri A
J Immunol Methods. 1981;41(1):95-103. doi: 10.1016/0022-1759(81)90277-5.
A rapid and sensitive solid-phase enzyme immunoassay (EIA) was developed for determination of serum antibodies, including addition of 4% (w/v) polyethylene glycol (PEG) to the diluent buffers. This modification accelerated the antigen-antibody reactions and made it possible to complete the assay within 2 h and to incubate at room temperature only. The enhancing effect of the polymer was particularly prominent in the second solid-phase immune reaction, the interaction between the antigen-bound antibody and the enzyme-labeled anti-immunoglobulin. The PEG-EIA procedure was successfully applied in the assay of antibodies to rubella, influenza A, herpes simplex and cytomegalo viruses, to Mycoplasma pneumoniae and Toxoplasma gondii.
开发了一种快速灵敏的固相酶免疫测定法（EIA）用于测定血清抗体，包括在稀释缓冲液中添加4%（w/v）聚乙二醇（PEG）。这种改进加速了抗原 - 抗体反应，使得能够在2小时内完成测定，并且仅在室温下孵育。聚合物的增强作用在第二次固相免疫反应中尤为显著，即抗原结合抗体与酶标记抗免疫球蛋白之间的相互作用。PEG - EIA方法已成功应用于风疹、甲型流感、单纯疱疹和巨细胞病毒、肺炎支原体和弓形虫抗体的测定。
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