Durban E M, Boettiger D
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3600-4. doi: 10.1073/pnas.78.6.3600.
Functionally differentiated chicken macrophages were derived by in vitro differentiation of embryonic yolk sac cells and were characterized by several macrophage-specific cell markers. Uniform, infected, virus-producing cultures were obtained after exposure of these macrophages to avian myoblastosis virus (AMV), avian myelocytomatosis virus (MC29), myeloblastosis-associated virus (MAV-2), and Prague strain of Rous sarcoma virus (PR-B RSV). Both AMV and MC29 induced morphological transformation typical of the in vivo leukemias induced by these virus strains. Analysis of the expression of macrophage-specific markers in these two transformed cell types demonstrated that different markers of the mature macrophage were suppressed by each virus, even though the parental cell immediately preceding the transformation event was a mature macrophage in both cases. Cells infected with PR-B RSV and MAV-2 showed no observable difference from uninfected macrophages in terms of morphological characteristics, growth rate, or expression of the differentiated functions of macrophages. Ths system provides demonstrations of a cell type that produces infectious, transforming RSV but fails to respond by functional alterations induced by the transforming gene, src.
通过胚胎卵黄囊细胞的体外分化获得了功能分化的鸡巨噬细胞，其具有多种巨噬细胞特异性细胞标志物。将这些巨噬细胞暴露于禽成肌细胞瘤病毒（AMV）、禽骨髓细胞瘤病毒（MC29）、成髓细胞瘤相关病毒（MAV-2）和劳氏肉瘤病毒布拉格株（PR-B RSV）后，获得了均匀、受感染且产生病毒的培养物。AMV和MC29均诱导了这些病毒株在体内诱导的白血病典型的形态转化。对这两种转化细胞类型中巨噬细胞特异性标志物表达的分析表明，尽管在转化事件之前的亲代细胞在两种情况下都是成熟巨噬细胞，但每种病毒都抑制了成熟巨噬细胞的不同标志物。感染PR-B RSV和MAV-2的细胞在形态特征、生长速率或巨噬细胞分化功能的表达方面与未感染的巨噬细胞没有明显差异。该系统证明了一种细胞类型，它产生具有传染性的、转化性的RSV，但不会因转化基因src诱导的功能改变而产生反应。
