与禽成髓细胞白血病相关的前病毒基因组的鉴定。

Souza L M, Komaromy M C, Baluda M A

Proc Natl Acad Sci U S A. 1980 May;77(5):3004-8. doi: 10.1073/pnas.77.5.3004.

We have identified and isolated a presumptive leukemogenic provirus from myeloblasts of a chicken in which leukemia had been induced by avian myeloblastosis virus (AMV). Leukemic myeloblasts isolated from peripheral blood or from converted yolk sac cultures of various strains of chickens, regardless of the endogenous proviral content or AMV pseudotype used for infection, contain an EcoRI 2.2-megadalton (MDaI) and a HindIII 2.6-MDal proviral fragment. A proviral genome flanked by chicken DNA sequences on either side and containing both the EcoRI 2.2-MDal and the HindIII 2.6-MDal fragments was inserted by molecular recombination into lambda phage Charon 4A and then cloned. This presumptive AMV proviral genome has a mass of approximately 4.9 MDal and contains terminal redundancies with respect to 3' viral RNA sequences.

我们已从一只由禽成髓细胞瘤病毒（AMV）诱发白血病的鸡的成髓细胞中鉴定并分离出一种假定的致白血病前病毒。从不同品系鸡的外周血或转化的卵黄囊培养物中分离出的白血病成髓细胞，无论其内源前病毒含量如何，也无论用于感染的AMV假型如何，均含有一个EcoRI 2.2兆道尔顿（MDal）和一个HindIII 2.6-MDal的前病毒片段。一个两侧侧翼均为鸡DNA序列且包含EcoRI 2.2-MDal和HindIII 2.6-MDal片段的前病毒基因组通过分子重组插入λ噬菌体Charon 4A中，然后进行克隆。这个假定的AMV前病毒基因组质量约为4.9 MDal，并且相对于3'病毒RNA序列含有末端冗余。