谷胱甘肽和尿苷5'-二磷酸葡糖醛酸对沙门氏菌/微粒体试验中苯并[a]芘和黄曲霉毒素B1致突变性的影响。
Effect of glutathione and uridine 5'-diphosphoglucuronic acid on mutagenesis by benzo[a]pyrene and aflatoxin B1 in the Salmonella/microsome assay.
作者信息
Malaveille C, Brun G, Hautefeuille A, Bartsch H
出版信息
Mutat Res. 1981 Aug;83(1):15-24. doi: 10.1016/0027-5107(81)90067-1.
In the Salmonella/microsome plate or liquid assay, the addition of glutathione (GSH) and uridine 5'-diphosphoglucuronic acid (UDPGA), both cofactors for GSH-S-transferases or UDPGA-transferases, altered the rat-liver microsome-mediated mutagenesis of benzo[a]pyrene (BP) and aflatoxin B1 (AFB). With either BP or AFB, an increased, unchanged or decreased number of revertant colonies of S. typhimurium was observed, depending on the substrate concentration, the source of rat-liver 9000 X g supernatant (S9), the time of incubation and the type of mutagenicity test (liquid or plate assay). Several factors responsible for quantitative changes in the pattern of BP and AFB metabolites under various assay conditions in vitro, which alter the overall mutagenic activity of the parent compound, are discussed.
在沙门氏菌/微粒体平板或液体试验中，添加谷胱甘肽（GSH）和尿苷5'-二磷酸葡糖醛酸（UDPGA）这两种谷胱甘肽-S-转移酶或UDPGA-转移酶的辅因子，改变了大鼠肝微粒体介导的苯并[a]芘（BP）和黄曲霉毒素B1（AFB）的诱变作用。对于BP或AFB，观察到鼠伤寒沙门氏菌回复菌落数量增加、不变或减少，这取决于底物浓度、大鼠肝9000×g上清液（S9）的来源、孵育时间和致突变性试验类型（液体或平板试验）。讨论了在体外各种试验条件下导致BP和AFB代谢产物模式发生定量变化的几个因素，这些因素改变了母体化合物的总体诱变活性。
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