Felber B K, Gerber-Huber S, Meier C, May F E, Westley B, Weber R, Ryffel G U
Nucleic Acids Res. 1981 Jun 11;9(11):2455-74. doi: 10.1093/nar/9.11.2455.
The disappearance of defined restriction fragments of the beta 1-globin, an albumin and the A1 vitellogenin gene was quantitated after DNase I digestion and expressed by a sensitivity factor defined by a mathematical model. Analysis of naked DNA showed that the gene fragments have similar but not identical sensitivity factors. DNase I digestion of chromatin revealed for the same gene fragments sensitivity factors differing over a much wilder range. This is correlated to the activity of the genes analyzed: the beta 1-globin gene fragment is more sensitive to DNase I in chromatin of erythrocytes compared to hepatocytes whereas the albumin gene fragment is more sensitive to DNase I in chromatin of hepatocytes. The A1 vitellogenin gene has the same DNase I sensitivity in both cell types. Comparing the DNase I sensitivity of the three genes in their inactive state we suggest that different chromatin conformations may exist for inactive genes.
在DNA酶I消化后，对β1-珠蛋白、一种白蛋白和A1卵黄蛋白原基因的特定限制性片段的消失进行了定量，并通过一个数学模型定义的敏感因子来表示。对裸露DNA的分析表明，基因片段具有相似但不完全相同的敏感因子。染色质的DNA酶I消化显示，相同的基因片段的敏感因子在更广泛的范围内存在差异。这与所分析基因的活性相关：与肝细胞相比，β1-珠蛋白基因片段在红细胞染色质中对DNA酶I更敏感，而白蛋白基因片段在肝细胞染色质中对DNA酶I更敏感。A1卵黄蛋白原基因在两种细胞类型中对DNA酶I的敏感性相同。比较这三个基因在非活性状态下对DNA酶I的敏感性，我们认为非活性基因可能存在不同的染色质构象。
