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有限的脱氧核糖核酸酶I切口作为基因构象的一种探测方法。

Limited DNase I nicking as a probe of gene conformation.

作者信息

Zasloff M, Camerini-Otero R D

出版信息

Proc Natl Acad Sci U S A. 1980 Apr;77(4):1907-11. doi: 10.1073/pnas.77.4.1907.

DOI:10.1073/pnas.77.4.1907
PMID:6929527
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC348618/
Abstract

We have extended the use of pancreatic DNase I as a probe of chromatin structure by exploring the accessibility of an active gene to the introduction of the first single-stranded nick. We show by a target analysis that the beta-globin gene is about 25-fold more sensitive to single-site nicking than is an average sequence in the chicken erythrocyte nucleus or the nontranscribed albumin gene. The sites of initial DNase I nicking are shown to cluster within the transcribed sequence of the beta-globin gene.

摘要

我们通过探索一个活性基因对首个单链切口引入的可及性，扩展了胰腺脱氧核糖核酸酶I（pancreatic DNase I）作为染色质结构探针的用途。我们通过靶点分析表明，β-珠蛋白基因对单一位点切口的敏感性比鸡红细胞核中的平均序列或非转录的白蛋白基因高约25倍。初始脱氧核糖核酸酶I切口的位点显示聚集在β-珠蛋白基因的转录序列内。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/293c/348618/24958b9b99ce/pnas00667-0224-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/293c/348618/2d620456d4f6/pnas00667-0223-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/293c/348618/45270eb945c9/pnas00667-0223-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/293c/348618/e45e08060e76/pnas00667-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/293c/348618/dd395eeef2ba/pnas00667-0224-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/293c/348618/24958b9b99ce/pnas00667-0224-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/293c/348618/2d620456d4f6/pnas00667-0223-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/293c/348618/45270eb945c9/pnas00667-0223-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/293c/348618/e45e08060e76/pnas00667-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/293c/348618/dd395eeef2ba/pnas00667-0224-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/293c/348618/24958b9b99ce/pnas00667-0224-c.jpg

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1
Limited DNase I nicking as a probe of gene conformation.有限的脱氧核糖核酸酶I切口作为基因构象的一种探测方法。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1907-11. doi: 10.1073/pnas.77.4.1907.
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Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin.分离出一类负责赋予珠蛋白染色质对核酸酶I敏感结构的核蛋白亚类。
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Digestion of the chicken beta-globin gene chromatin with micrococcal nuclease reveals the presence of an altered nucleosomal array characterized by an atypical ladder of DNA fragments.用微球菌核酸酶消化鸡β-珠蛋白基因染色质，发现存在一种改变的核小体阵列，其特征是DNA片段呈现非典型的梯状条带。
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引用本文的文献

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Mol Cell Biol. 1983 Mar;3(3):399-409. doi: 10.1128/mcb.3.3.399-409.1983.
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Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.利用分析型琼脂糖-溴化乙锭电泳检测副流感嗜血杆菌中的两种限制性内切酶活性。
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Estrogen induces tissue specific changes in the chromatin conformation of the vitellogenin genes in Xenopus.雌激素诱导非洲爪蟾卵黄蛋白原基因染色质构象发生组织特异性变化。
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Quantitation of DNase I sensitivity in Xenopus chromatin containing active and inactive globin, albumin and vitellogenin genes.非洲爪蟾染色质中含有活性和非活性珠蛋白、白蛋白及卵黄蛋白原基因的DNA酶I敏感性定量分析。
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Nucleosomal packaging of the thymidine kinase gene of herpes simplex virus transferred into mouse cells: an actively expressed single-copy gene.转移至小鼠细胞中的单纯疱疹病毒胸苷激酶基因的核小体包装：一个活跃表达的单拷贝基因。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5079-83. doi: 10.1073/pnas.77.9.5079.
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A structure of potentially active and inactive genes of chicken erythrocyte chromatin upon decondensation.鸡红细胞染色质解聚时潜在活性和非活性基因的结构
Nucleic Acids Res. 1988 Sep 12;16(17):8555-69. doi: 10.1093/nar/16.17.8555.
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
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Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.从输卵管细胞核中对转录活性卵清蛋白基因进行选择性消化。
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3966-70. doi: 10.1073/pnas.73.11.3966.
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Nucleotide sequence of the rightward operator of phage lambda.噬菌体λ右向操纵基因的核苷酸序列。
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Chromosomal subunits in active genes have an altered conformation.活跃基因中的染色体亚基具有改变的构象。
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A physical map of the DNA regions flanking the rabbit beta-globin gene.兔β-珠蛋白基因侧翼DNA区域的物理图谱。
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Renaturation kinetics of cDNA complementary to cytoplamic polyadenylated RNA from rainbow trout testis. Accessibility of transcribed genes to pancreatic DNase.虹鳟鱼睾丸细胞质多聚腺苷酸化RNA互补cDNA的复性动力学。转录基因对胰腺DNA酶的可及性。
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Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.T7 DNA限制性片段分析及通过中性和碱性凝胶电泳测定分子量
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Cloning of a double-stranded cDNA that codes for a portion of chicken preproalbumin. A general method for isolating a specific DNA sequence from partially purified mRNA.编码鸡前清蛋白一部分的双链cDNA的克隆。从部分纯化的mRNA中分离特定DNA序列的通用方法。
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