鼠伤寒沙门氏菌组氨酸转运操纵子启动子突变分析：利用杂交M13噬菌体进行克隆、转化及测序
Analysis of promoter mutations in the histidine transport operon of Salmonella typhimurium: use of hybrid M13 bacteriophages for cloning, transformation, and sequencing.
作者信息
Lee G S, Ames G F
出版信息
J Bacteriol. 1984 Sep;159(3):1000-5. doi: 10.1128/jb.159.3.1000-1005.1984.
Abstract
Mutations that cause an increased level of expression of the histidine transport operon were isolated and characterized genetically. Five independently isolated promoter-up mutations were transferred to an M13 hybrid phage that carries the histidine transport operon, and their nucleotide sequences were determined. For all five mutations, the change was the same as the one previously determined for promoter-up mutation dhuA1: a C-to-T change in the Pribnow box rendered this region more homologous to the consensus sequence. Methods for enabling Salmonella typhimurium to support growth of M13 phage effectively and for easy transfer of chromosomal mutations onto the hybrid phage are presented.
摘要
分离出导致组氨酸转运操纵子表达水平升高的突变，并对其进行遗传学特征分析。将五个独立分离的启动子上调突变转移到携带组氨酸转运操纵子的M13杂交噬菌体中，并测定其核苷酸序列。对于所有五个突变，其变化与先前确定的启动子上调突变dhuA1相同：Pribnow框中的C到T变化使该区域与共有序列更同源。本文介绍了使鼠伤寒沙门氏菌有效支持M13噬菌体生长以及将染色体突变轻松转移到杂交噬菌体上的方法。
相似文献
1
Analysis of promoter mutations in the histidine transport operon of Salmonella typhimurium: use of hybrid M13 bacteriophages for cloning, transformation, and sequencing.鼠伤寒沙门氏菌组氨酸转运操纵子启动子突变分析：利用杂交M13噬菌体进行克隆、转化及测序
J Bacteriol. 1984 Sep;159(3):1000-5. doi: 10.1128/jb.159.3.1000-1005.1984.
2
Physical map of the Salmonella typhimurium histidine transport operon: correlation with the genetic map.鼠伤寒沙门氏菌组氨酸转运操纵子的物理图谱：与遗传图谱的相关性。
J Bacteriol. 1981 Aug;147(2):401-9. doi: 10.1128/jb.147.2.401-409.1981.
3
Use of M13mp phages to study gene regulation, structure and function: cloning and recombinational analysis of genes of the Salmonella typhimurium histidine operon.利用M13mp噬菌体研究基因调控、结构与功能：鼠伤寒沙门氏菌组氨酸操纵子基因的克隆与重组分析
Gene. 1983 Dec;26(2-3):147-58. doi: 10.1016/0378-1119(83)90184-1.
4
The DNA sequence of the promoter-attenuator of the ilvGEDA operon of Salmonella typhimurium.鼠伤寒沙门氏菌ilvGEDA操纵子启动子-弱化子的DNA序列。
Nucleic Acids Res. 1981 Jul 24;9(14):3419-32. doi: 10.1093/nar/9.14.3419.
5
Construction of an M13 histidine-transducing phage: a single-stranded cloning vehicle with one EcoRI site.构建M13组氨酸转导噬菌体：一种具有单个EcoRI位点的单链克隆载体。
Gene. 1979 Feb;5(2):127-39. doi: 10.1016/0378-1119(79)90098-2.
6
Mutational analysis of the histidine operon promoter of Salmonella typhimurium.鼠伤寒沙门氏菌组氨酸操纵子启动子的突变分析。
J Bacteriol. 1989 Nov;171(11):6330-7. doi: 10.1128/jb.171.11.6330-6337.1989.
7
Isolation of F' plasmids carrying a portion of the Salmonella typhimurium histidine transport operon.携带鼠伤寒沙门氏菌组氨酸转运操纵子一部分的F'质粒的分离。
J Bacteriol. 1984 Mar;157(3):697-702. doi: 10.1128/jb.157.3.697-702.1984.
8
A mutational hot-spot in the hisM gene of the histidine transport operon in Salmonella typhimurium is due to deletion of repeated sequences and results in an altered specificity of transport.鼠伤寒沙门氏菌组氨酸转运操纵子的hisM基因中的一个突变热点是由于重复序列的缺失，导致转运特异性改变。
Mol Gen Genet. 1985;200(3):493-6. doi: 10.1007/BF00425737.
9
Complete nucleotide sequence and identification of membrane components of the histidine transport operon of S. typhimurium.鼠伤寒沙门氏菌组氨酸转运操纵子的完整核苷酸序列及膜成分鉴定
Nature. 1982 Aug 19;298(5876):723-7. doi: 10.1038/298723a0.
10
Isolation and characterization of lac fusions to two nitrogen-regulated promoters.与两个氮调节启动子的乳糖融合物的分离与鉴定。
Mol Gen Genet. 1984;195(1-2):219-27. doi: 10.1007/BF00332750.
引用本文的文献
1
Phage-delivered CRISPR-Cas9 for strain-specific depletion and genomic deletions in the gut microbiome.噬菌体递送 CRISPR-Cas9 用于肠道微生物组中特定菌株的耗竭和基因组缺失。
Cell Rep. 2021 Nov 2;37(5):109930. doi: 10.1016/j.celrep.2021.109930.
2
D-histidine utilization in Salmonella typhimurium is controlled by the leucine-responsive regulatory protein (Lrp).鼠伤寒沙门氏菌中D-组氨酸的利用受亮氨酸应答调节蛋白（Lrp）的控制。
J Bacteriol. 1996 Jan;178(2):327-31. doi: 10.1128/jb.178.2.327-331.1996.
3
A mutational hot-spot in the hisM gene of the histidine transport operon in Salmonella typhimurium is due to deletion of repeated sequences and results in an altered specificity of transport.鼠伤寒沙门氏菌组氨酸转运操纵子的hisM基因中的一个突变热点是由于重复序列的缺失，导致转运特异性改变。
Mol Gen Genet. 1985;200(3):493-6. doi: 10.1007/BF00425737.
4
An inducible pathway is required for mutagenesis in Salmonella typhimurium LT2.鼠伤寒沙门氏菌LT2中的诱变需要一条可诱导途径。
J Bacteriol. 1987 Jun;169(6):2885-8. doi: 10.1128/jb.169.6.2885-2888.1987.
5
Linkage map of Salmonella typhimurium, edition VII.鼠伤寒沙门氏菌连锁图谱，第七版。
Microbiol Rev. 1988 Dec;52(4):485-532. doi: 10.1128/mr.52.4.485-532.1988.
6
Nitrogen regulation in Salmonella typhimurium. Identification of an ntrC protein-binding site and definition of a consensus binding sequence.鼠伤寒沙门氏菌中的氮调节。ntrC蛋白结合位点的鉴定及共有结合序列的定义。
EMBO J. 1985 Feb;4(2):539-47. doi: 10.1002/j.1460-2075.1985.tb03662.x.
7
Gene replacement and retrieval with recombinant M13mp bacteriophages.利用重组M13mp噬菌体进行基因置换和检索。
J Bacteriol. 1989 Jan;171(1):538-46. doi: 10.1128/jb.171.1.538-546.1989.
本文引用的文献
1
Complete nucleotide sequence and identification of membrane components of the histidine transport operon of S. typhimurium.鼠伤寒沙门氏菌组氨酸转运操纵子的完整核苷酸序列及膜成分鉴定
Nature. 1982 Aug 19;298(5876):723-7. doi: 10.1038/298723a0.
2
Regulatory regions of two transport operons under nitrogen control: nucleotide sequences.氮控制下两个转运操纵子的调控区域：核苷酸序列
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1083-7. doi: 10.1073/pnas.79.4.1083.
3
Regulation of high-affinity leucine transport in Escherichia coli.大肠杆菌中高亲和力亮氨酸转运的调控
J Supramol Struct. 1980;14(4):527-37. doi: 10.1002/jss.400140410.
4
Sequence and properties of operator mutations in the bio operon of Escherichia coli.大肠杆菌生物操纵子中操纵基因突变的序列与特性
Gene. 1981 Jan-Feb;13(1):89-102. doi: 10.1016/0378-1119(81)90046-9.
5
DNA sequence analysis of prm-mutations of coliphage lambda.大肠杆菌噬菌体λ的prm突变的DNA序列分析
Gene. 1980 Nov;11(3-4):197-205. doi: 10.1016/0378-1119(80)90060-8.
6
Cloning of the histidine transport genes from Salmonella typhimurium and characterization of an analogous transport system in Escherichia coli.鼠伤寒沙门氏菌组氨酸转运基因的克隆及大肠杆菌中类似转运系统的特性研究。
J Supramol Struct. 1980;13(1):117-30. doi: 10.1002/jss.400130111.
7
Repetitive extragenic palindromic sequences: a major component of the bacterial genome.重复基因外回文序列：细菌基因组的主要组成部分。
Cell. 1984 Jul;37(3):1015-26. doi: 10.1016/0092-8674(84)90436-7.
8
The nif promoters of Klebsiella pneumoniae have a characteristic primary structure.肺炎克雷伯菌的nif启动子具有独特的一级结构。
Cell. 1983 Sep;34(2):665-71. doi: 10.1016/0092-8674(83)90399-9.
9
Two periplasmic transport proteins which interact with a common membrane receptor show extensive homology: complete nucleotide sequences.两种与共同膜受体相互作用的周质转运蛋白显示出广泛的同源性：完整的核苷酸序列。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6038-42. doi: 10.1073/pnas.78.10.6038.
10
Attenuation in amino acid biosynthetic operons.氨基酸生物合成操纵子中的衰减作用。
Annu Rev Genet. 1982;16:113-34. doi: 10.1146/annurev.ge.16.120182.000553.
Zotero 插件