鼠伤寒沙门氏菌中乙酰羟酸合酶I和II缺陷型突变体。
Salmonella typhimurium mutants defective in acetohydroxy acid synthases I and II.
作者信息
Shaw K J, Berg C M, Sobol T J
出版信息
J Bacteriol. 1980 Mar;141(3):1258-63. doi: 10.1128/jb.141.3.1258-1263.1980.
Abstract
An analysis of transposon-induced mutants shows that Salmonella typhimurium possesses two major isozymes of acetohydroxy acid synthase, the enzymes which mediate the first common step in isoleucine and valine biosynthesis. A third (minor) acetohydroxy acid synthase is present, but its significance in isoleucine and valine synthesis may be negligible. Mutants defective in acetohydroxy acid synthase II (ilvG::Tn10) require isoleucine, alpha-ketobutyrate, or threonine for growth, a mutant defective in acetohydroxy acid synthase I (ilvB::Tn5) is a prototroph, and a double mutant (ilvG::Tn10 ilvB::Tn5) requires isoleucine plus valine for growth.
摘要
对转座子诱导突变体的分析表明，鼠伤寒沙门氏菌拥有两种主要的乙酰羟酸合酶同工酶，这些酶介导异亮氨酸和缬氨酸生物合成的第一步共同反应。还存在第三种（次要的）乙酰羟酸合酶，但其在异亮氨酸和缬氨酸合成中的意义可能微不足道。乙酰羟酸合酶II缺陷型突变体（ilvG::Tn10）生长需要异亮氨酸、α-酮丁酸或苏氨酸，乙酰羟酸合酶I缺陷型突变体（ilvB::Tn5）是原养型，而双突变体（ilvG::Tn10 ilvB::Tn5）生长需要异亮氨酸加缬氨酸。
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