Mahjoubfar Ata, Chen Claire, Niazi Kayvan R, Rabizadeh Shahrooz, Jalali Bahram
Department of Electrical Engineering, University of California, Los Angeles, California 90095 USA ; California NanoSystems Institute, Los Angeles, California 90095 USA.
Biomed Opt Express. 2013 Aug 12;4(9):1618-25. doi: 10.1364/BOE.4.001618. eCollection 2013.
Flow cytometry is a powerful tool for cell counting and biomarker detection in biotechnology and medicine especially with regards to blood analysis. Standard flow cytometers perform cell type classification both by estimating size and granularity of cells using forward- and side-scattered light signals and through the collection of emission spectra of fluorescently-labeled cells. However, cell surface labeling as a means of marking cells is often undesirable as many reagents negatively impact cellular viability or provide activating/inhibitory signals, which can alter the behavior of the desired cellular subtypes for downstream applications or analysis. To eliminate the need for labeling, we introduce a label-free imaging-based flow cytometer that measures size and cell protein concentration simultaneously either as a stand-alone instrument or as an add-on to conventional flow cytometers. Cell protein concentration adds a parameter to cell classification, which improves the specificity and sensitivity of flow cytometers without the requirement of cell labeling. This system uses coherent dispersive Fourier transform to perform phase imaging at flow speeds as high as a few meters per second.
流式细胞术是生物技术和医学领域中用于细胞计数和生物标志物检测的强大工具，尤其在血液分析方面。标准流式细胞仪通过利用前向和侧向散射光信号估计细胞大小和粒度，并通过收集荧光标记细胞的发射光谱来进行细胞类型分类。然而，细胞表面标记作为标记细胞的一种手段通常并不理想，因为许多试剂会对细胞活力产生负面影响或提供激活/抑制信号，这可能会改变下游应用或分析中所需细胞亚型的行为。为了消除标记的需求，我们引入了一种基于无标记成像的流式细胞仪，它可以作为独立仪器或作为传统流式细胞仪的附加装置同时测量细胞大小和细胞蛋白质浓度。细胞蛋白质浓度为细胞分类增加了一个参数，可以提高流式细胞仪的特异性和灵敏度，而无需进行细胞标记。该系统使用相干色散傅里叶变换在高达每秒几米的流速下进行相位成像。
