Pearson W R, Morrow J F
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4016-20. doi: 10.1073/pnas.78.7.4016.
Two of the four repeated DNA sequences near the 5' end of the silk fibroin gene hybridize with discrete-length families of repeated DNA. These two families comprise 0.5% of the animal's genome. A repeated sequence with a conserved length has also been found in the short class of moderately repeated sequences in the sea urchin. The discrete length, interspersion, and sequence fidelity of these moderately repeated sequences suggests that each has been multiplied as a discrete unit. Thus, transposition mechanisms may be responsible for the multiplication and dispersion of a large class of repeated sequences in phylogenetically diverse eukaryotic genomes. The repeat we have studied in most detail differs from previously described eukaryotic transposable elements: it is much shorter (1300 base pairs) and does not have terminal repetitions detectable by DNA hybridization. A simple technique for identifying such discrete-length repeated sequences is described.
丝心蛋白基因5'端附近的四个重复DNA序列中有两个与重复DNA的离散长度家族杂交。这两个家族占动物基因组的0.5%。在海胆中等重复序列的短类中也发现了一个具有保守长度的重复序列。这些中等重复序列的离散长度、散布情况和序列保真度表明，每个序列都是作为一个离散单元进行倍增的。因此，转座机制可能是造成系统发育上不同的真核生物基因组中一大类重复序列倍增和散布的原因。我们研究得最详细的重复序列与先前描述的真核转座元件不同：它要短得多（1300个碱基对），并且通过DNA杂交检测不到末端重复。本文描述了一种识别此类离散长度重复序列的简单技术。
