Roeder G S, Farabaugh P J, Chaleff D T, Fink G R
Science. 1980 Sep 19;209(4463):1375-80. doi: 10.1126/science.6251544.
Two unstable mutations at the his4 locus of yeast are due to the insertion of the transposable elements Ty912 and Ty917 into the his4 regulatory region. The two transposons are related, one being derived from the other by a substitution of 4000 base pairs of DNA. Element Ty912 includes identical terminal repeats, whereas the terminal repeats of Ty917 are not identical. Transposition of Ty912 or Ty917 generates 5-base-pair duplications of the target DNA at either end of the element. Expression and reversion of a his4 gene containing Ty912 or Ty917 is controlled by three unlinked regulatory genes. The properties of these regulatory genes are similar to those described for the controlling elements in maize.
酵母 his4 基因座上的两个不稳定突变是由于转座元件 Ty912 和 Ty917 插入到 his4 调控区域所致。这两个转座子相关，其中一个是另一个通过替换 4000 个碱基对的 DNA 衍生而来。元件 Ty912 包含相同的末端重复序列，而 Ty917 的末端重复序列并不相同。Ty912 或 Ty917 的转座在元件两端产生靶 DNA 的 5 个碱基对重复。含有 Ty912 或 Ty917 的 his4 基因的表达和回复受三个不连锁的调控基因控制。这些调控基因的特性与玉米中描述的控制元件相似。
