Tracy S, Smith R A
J Gen Virol. 1981 Jul;55(Pt 1):193-9. doi: 10.1099/0022-1317-55-1-193.
RNAs from 11 strains of the three serotypes of poliovirus were isolated from infected HeLa cells and hybridized with representative, radioactive complementary DNA (cDNA) from either poliovirus type 1 or type 2. Hybridization and thermal denaturation were analysed by hydroxyapatite chromatography, cDNA to either poliovirus type 1 or 2 is competent to detect about 70 to 80% of the sequences in RNAs from heterotypic strains and more than 02% of the RNA sequences in homotypic strains. The three polio serotype genomes appear to have diverged from each other by about 20%. Both poliovirus type 1 and type 2 representative cDNA can be used for the detection of poliovirus genomic sequences in RNA isolated from tissue.
从感染的HeLa细胞中分离出三种血清型脊髓灰质炎病毒11个毒株的RNA，并与脊髓灰质炎病毒1型或2型的代表性放射性互补DNA（cDNA）杂交。通过羟基磷灰石柱层析分析杂交和热变性情况，1型或2型脊髓灰质炎病毒的cDNA能够检测出异型毒株RNA中约70%至80%的序列以及同型毒株RNA中超过92%的序列。三种脊髓灰质炎病毒血清型基因组似乎彼此之间有大约20%的差异。脊髓灰质炎病毒1型和2型的代表性cDNA均可用于检测从组织中分离出的RNA中的脊髓灰质炎病毒基因组序列。
