通过cDNA:RNA杂交法对3型脊髓灰质炎病毒株基因组进行分子克隆
Molecular cloning of the genomes of poliovirus type 3 strains by the cDNA: RNA hybrid method.
作者信息
Stanway G, Mountford R C, Cox S D, Schild G C, Minor P D, Almond J W
出版信息
Arch Virol. 1984;81(1-2):67-78. doi: 10.1007/BF01309297.
PMID:6331350
Abstract
The genomes of two neurovirulent strains of poliovirus type 3, wild type P3/Leon/37 and a vaccine revertant P3/119/70, have been cloned in E. coli. The cDNA: RNA hybrid method used was efficient and may have wide applicability for cDNA cloning. Overlapping clones spanning the entire genome were obtained for each strain. These have been used to produce full-length DNA copies of the two genomes each within a single plasmid.
摘要
3型脊髓灰质炎病毒的两种神经毒性毒株，野生型P3/Leon/37和一种疫苗回复株P3/119/70的基因组已在大肠杆菌中克隆。所使用的cDNA:RNA杂交方法效率高，可能在cDNA克隆中有广泛的适用性。为每个毒株获得了覆盖整个基因组的重叠克隆。这些克隆已被用于在单个质粒中产生这两种基因组的全长DNA拷贝。
Zotero 插件