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3型脊髓灰质炎病毒：基因组的分子克隆以及编码蛋白酶和聚合酶蛋白区域的核苷酸序列

Poliovirus type 3: molecular cloning of the genome and nucleotide sequence of the region encoding the protease and polymerase proteins.

作者信息

Cann A J, Stanway G, Hauptmann R, Minor P D, Schild G C, Clarke L D, Mountford R C, Almond J W

出版信息

Nucleic Acids Res. 1983 Mar 11;11(5):1267-81. doi: 10.1093/nar/11.5.1267.

DOI:10.1093/nar/11.5.1267
PMID:6298739
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC325795/
Abstract

Overlapping cDNA clones representing the entire genome of poliovirus type 3 have been prepared in E. coli by two separate methods. Cloning of RNA . cDNA hybrids produced a more comprehensive set of clones with generally larger cDNA inserts than cloning of double - stranded cDNA. A restriction map of the entire genome and the nucleotide sequence of 2003 bases from the 3' terminus, comprising the region encoding the protease and polymerase proteins, are presented.

摘要

通过两种不同方法在大肠杆菌中制备了代表3型脊髓灰质炎病毒全基因组的重叠cDNA克隆。与双链cDNA克隆相比，RNA.cDNA杂种的克隆产生了一套更完整的克隆，其cDNA插入片段通常更大。本文给出了整个基因组的限制性图谱以及来自3'末端的2003个碱基的核苷酸序列，该区域包含编码蛋白酶和聚合酶蛋白的区域。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c41/325795/dce591f97f1e/nar00350-0070-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c41/325795/e45df39bb954/nar00350-0068-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c41/325795/dce591f97f1e/nar00350-0070-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c41/325795/e45df39bb954/nar00350-0068-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c41/325795/dce591f97f1e/nar00350-0070-a.jpg

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引用本文的文献

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Molecular cloning of the genomes of poliovirus type 3 strains by the cDNA: RNA hybrid method.通过cDNA:RNA杂交法对3型脊髓灰质炎病毒株基因组进行分子克隆
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Comparison of the complete nucleotide sequences of the genomes of the neurovirulent poliovirus P3/Leon/37 and its attenuated Sabin vaccine derivative P3/Leon 12a1b.神经毒性脊髓灰质炎病毒P3/Leon/37及其减毒的萨宾疫苗衍生物P3/Leon 12a1b的基因组完整核苷酸序列比较。
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1539-43. doi: 10.1073/pnas.81.5.1539.
3
The nucleotide sequence of poliovirus type 3 leon 12 a1b: comparison with poliovirus type 1.

本文引用的文献

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A new computer method for the storage and manipulation of DNA gel reading data.一种用于存储和处理DNA凝胶读数数据的新型计算机方法。
Nucleic Acids Res. 1980 Aug 25;8(16):3673-94. doi: 10.1093/nar/8.16.3673.
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Isolation of developmentally regulated genes from Dictyostelium.从盘基网柄菌中分离发育调控基因。
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Trans-complementable copy-number mutants of plasmid ColE1.质粒ColE1的反式可互补拷贝数突变体
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Nucleic Acids Res. 1983 Aug 25;11(16):5629-43. doi: 10.1093/nar/11.16.5629.
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Sequence of the membrane protein gene from avian coronavirus IBV.禽冠状病毒传染性支气管炎病毒（IBV）膜蛋白基因序列
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The complete nucleotide sequence of a common cold virus: human rhinovirus 14.一种普通感冒病毒——人鼻病毒14型的完整核苷酸序列。
Nucleic Acids Res. 1984 Oct 25;12(20):7859-75. doi: 10.1093/nar/12.20.7859.
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Reversion to neurovirulence of the live-attenuated Sabin type 3 oral poliovirus vaccine.减毒活疫苗萨宾3型口服脊髓灰质炎病毒的神经毒力回复
Nucleic Acids Res. 1984 Oct 25;12(20):7787-92. doi: 10.1093/nar/12.20.7787.
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The sequence of the coxsackievirus A 21 polymerase gene indicates a remarkably close relationship to the polioviruses. Brief report.柯萨奇病毒A21聚合酶基因序列显示与脊髓灰质炎病毒有非常密切的关系。简报。
Arch Virol. 1987;94(1-2):141-7. doi: 10.1007/BF01313732.
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Construction of poliovirus intertypic recombinants by use of cDNA.利用互补DNA构建脊髓灰质炎病毒的型间重组体。
J Virol. 1986 Mar;57(3):1187-90. doi: 10.1128/JVI.57.3.1187-1190.1986.
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Molecular cloning and partial characterization of the coxsackievirus B3 genome. Brief report.
Arch Virol. 1985;85(1-2):157-63. doi: 10.1007/BF01317016.
10
Rotavirus neutralizing protein VP7: antigenic determinants investigated by sequence analysis and peptide synthesis.轮状病毒中和蛋白VP7：通过序列分析和肽合成研究的抗原决定簇
J Virol. 1985 Jun;54(3):791-7. doi: 10.1128/JVI.54.3.791-797.1985.
Nature. 1980 Jan 10;283(5743):216-8. doi: 10.1038/283216a0.
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Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence.通过用根据病毒核苷酸序列预测的化学合成肽进行免疫接种来预防口蹄疫。
Nature. 1982 Jul 1;298(5869):30-3. doi: 10.1038/298030a0.
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Heavy chain variable region contribution to the NPb family of antibodies: somatic mutation evident in a gamma 2a variable region.重链可变区对NPb抗体家族的贡献：γ2a可变区中明显的体细胞突变。
Cell. 1981 Jun;24(3):625-37. doi: 10.1016/0092-8674(81)90089-1.
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Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome.减毒脊髓灰质炎病毒萨宾1型毒株基因组的完整核苷酸序列。
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