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1
Nucleotide sequence of an external transcribed spacer in Xenopus laevis rDNA: sequences flanking the 5' and 3' ends of 18S rRNA are non-complementary.非洲爪蟾核糖体DNA中外转录间隔区的核苷酸序列：18S核糖体RNA 5'和3'末端侧翼的序列不互补。
Nucleic Acids Res. 1982 Apr 10;10(7):2387-98. doi: 10.1093/nar/10.7.2387.
2
The external transcribed spacer and preceding region of Xenopus borealis rDNA: comparison with the corresponding region of Xenopus laevis rDNA.非洲爪蟾核糖体DNA的外部转录间隔区及前导区域：与非洲爪蟾核糖体DNA相应区域的比较。
Nucleic Acids Res. 1983 Dec 10;11(23):8183-96. doi: 10.1093/nar/11.23.8183.
3
Nucleotide sequence encoding the 5' end of Xenopus laevis 18S rRNA.编码非洲爪蟾18S核糖体RNA 5'端的核苷酸序列。
Nucleic Acids Res. 1980 Jul 11;8(13):2871-84. doi: 10.1093/nar/8.13.2871.
4
Structure of mouse rRNA precursors. Complete sequence and potential folding of the spacer regions between 18S and 28S rRNA.小鼠核糖体RNA前体的结构。18S与28S核糖体RNA之间间隔区的完整序列及潜在折叠。
Nucleic Acids Res. 1983 May 25;11(10):3375-91. doi: 10.1093/nar/11.10.3375.
5
Nucleotide sequence of the 18S-26S rRNA intergene region of the sea urchin.海胆18S - 26S rRNA基因间隔区的核苷酸序列。
Nucleic Acids Res. 1984 Feb 10;12(3):1737-47. doi: 10.1093/nar/12.3.1737.
6
Nucleotide sequence through the 18S-28S intergene region of a vertebrate ribosomal transcription unit.脊椎动物核糖体转录单位18S - 28S基因间隔区的核苷酸序列。
Nucleic Acids Res. 1980 Dec 20;8(24):5993-6005. doi: 10.1093/nar/8.24.5993.
7
Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis.非洲爪蟾核糖体基因单位中间隔DNA的序列组织。
Cell. 1979 May;17(1):19-31. doi: 10.1016/0092-8674(79)90291-5.
8
Nucleotide sequence of Xenopus borealis oocyte 5S DNA: comparison of sequences that flank several related eucaryotic genes.北方爪蟾卵母细胞5S DNA的核苷酸序列：几个相关真核基因侧翼序列的比较
Cell. 1978 Dec;15(4):1145-56. doi: 10.1016/0092-8674(78)90042-9.
9
Sequence organization of the spacer in the ribosomal genes of Xenopus clivii and Xenopus borealis.非洲爪蟾和北方爪蟾核糖体基因间隔区的序列组织
Nucleic Acids Res. 1981 Oct 24;9(20):5311-30. doi: 10.1093/nar/9.20.5311.
10
Multiple heterogeneities in the transcribed spacers of ribosomal DNA from Xenopus laevis.非洲爪蟾核糖体DNA转录间隔区的多种异质性
Nucleic Acids Res. 1983 Feb 11;11(3):629-46. doi: 10.1093/nar/11.3.629.

引用本文的文献

1
Patterns of major divergence between the internal transcribed spacers of ribosomal DNA in Xenopus borealis and Xenopus laevis, and of minimal divergence within ribosomal coding regions.北方爪蟾和非洲爪蟾核糖体DNA内部转录间隔区的主要差异模式，以及核糖体编码区内的最小差异。
EMBO J. 1983;2(3):443-8. doi: 10.1002/j.1460-2075.1983.tb01442.x.
2
Analysis of intergenic spacer transcripts suggests 'read-around' transcription of the extrachromosomal circular rDNA in Euglena gracilis.基因间间隔转录本分析表明纤细裸藻中染色体外环状rDNA存在“通读”转录。
Nucleic Acids Res. 2001 May 15;29(10):2191-8. doi: 10.1093/nar/29.10.2191.
3
The external transcribed spacer and preceding region of Xenopus borealis rDNA: comparison with the corresponding region of Xenopus laevis rDNA.非洲爪蟾核糖体DNA的外部转录间隔区及前导区域：与非洲爪蟾核糖体DNA相应区域的比较。
Nucleic Acids Res. 1983 Dec 10;11(23):8183-96. doi: 10.1093/nar/11.23.8183.
4
Location of the initial cleavage sites in mouse pre-rRNA.小鼠前体核糖体RNA中初始切割位点的位置
Mol Cell Biol. 1983 Aug;3(8):1501-10. doi: 10.1128/mcb.3.8.1501-1510.1983.
5
Nucleotide sequence of the 5'-terminal region of rat 18S ribosomal DNA.大鼠18S核糖体DNA 5′末端区域的核苷酸序列
Mol Gen Genet. 1983;191(3):427-9. doi: 10.1007/BF00425758.
6
Localization and structure of endonuclease cleavage sites involved in the processing of the rat 32S precursor to ribosomal RNA.参与大鼠核糖体RNA 32S前体加工的核酸内切酶切割位点的定位与结构
Biochem J. 1984 May 15;220(1):105-16. doi: 10.1042/bj2200105.
7
18S coding sequences in amplified ribosomal DNA from Xenopus laevis oocytes are highly homogeneous, unmethylated, and lack major open reading frames.非洲爪蟾卵母细胞扩增核糖体DNA中的18S编码序列高度同源、未甲基化且缺乏主要开放阅读框。
EMBO J. 1982;1(5):597-601. doi: 10.1002/j.1460-2075.1982.tb01214.x.
8
Nucleotide sequence of the 5'-terminal coding region for pre-rRNA and mature 17S rRNA in Tetrahymena thermophila rDNA.嗜热四膜虫核糖体DNA中前体核糖体RNA和成熟17S核糖体RNA的5'端编码区核苷酸序列。
Nucleic Acids Res. 1984 Jan 25;12(2):959-72. doi: 10.1093/nar/12.2.959.
9
The nucleotide sequence of an unusual non-transcribed spacer and its ancestor in the rDNA in Chironomus thummi.摇蚊（Chironomus thummi）核糖体DNA中一个不寻常的非转录间隔区及其祖先的核苷酸序列。
EMBO J. 1983;2(7):1177-83. doi: 10.1002/j.1460-2075.1983.tb01564.x.
10
The complete nucleotide sequence of the rat 18S ribosomal RNA gene and comparison with the respective yeast and frog genes.大鼠18S核糖体RNA基因的完整核苷酸序列及其与相应酵母和青蛙基因的比较。
Nucleic Acids Res. 1983 Jul 25;11(14):4879-90. doi: 10.1093/nar/11.14.4879.

本文引用的文献

1
The nucleotide sequence of the intergenic region between the 5.8S and 26S rRNA genes of the yeast ribosomal RNA operon. Possible implications for the interaction between 5.8S and 26S rRNA and the processing of the primary transcript.酵母核糖体RNA操纵子中5.8S和26S rRNA基因之间基因间区域的核苷酸序列。对5.8S和26S rRNA之间相互作用以及初级转录本加工的可能影响。
Nucleic Acids Res. 1981 Oct 10;9(19):4847-62. doi: 10.1093/nar/9.19.4847.
2
Nucleotide sequence of Xenopus laevis 18S ribosomal RNA inferred from gene sequence.从基因序列推断出的非洲爪蟾18S核糖体RNA的核苷酸序列。
Nature. 1981 May 21;291(5812):205-8. doi: 10.1038/291205a0.
3
Nucleotide sequence through the 18S-28S intergene region of a vertebrate ribosomal transcription unit.脊椎动物核糖体转录单位18S - 28S基因间隔区的核苷酸序列。
Nucleic Acids Res. 1980 Dec 20;8(24):5993-6005. doi: 10.1093/nar/8.24.5993.
4
Fine structure of ribosomal RNA. IV. Extraordinary evolutionary conservation in sequences that flank introns in rDNA.核糖体RNA的精细结构。IV. rDNA中内含子侧翼序列的异常进化保守性。
Nucleic Acids Res. 1980 Aug 25;8(16):3623-37. doi: 10.1093/nar/8.16.3623.
5
Nucleotide sequence encoding the 5' end of Xenopus laevis 18S rRNA.编码非洲爪蟾18S核糖体RNA 5'端的核苷酸序列。
Nucleic Acids Res. 1980 Jul 11;8(13):2871-84. doi: 10.1093/nar/8.13.2871.
6
Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
7
Properties of the ribosomal RNA precursor in Xenopus laevis; comparison to the precursor in mammals and in plants.非洲爪蟾核糖体RNA前体的特性；与哺乳动物和植物前体的比较。
J Mol Biol. 1969 Oct 28;45(2):353-66. doi: 10.1016/0022-2836(69)90110-7.
8
Secondary structure maps of ribosomal RNA and DNA. I. Processing of Xenopus laevis ribosomal RNA and structure of single-stranded ribosomal DNA.核糖体RNA和DNA的二级结构图谱。I. 非洲爪蟾核糖体RNA的加工及单链核糖体DNA的结构
J Mol Biol. 1974 Oct 25;89(2):379-95. doi: 10.1016/0022-2836(74)90526-9.
9
Processing of 45 s nucleolar RNA.45秒核仁RNA的加工
J Mol Biol. 1970 Jan 28;47(2):169-78. doi: 10.1016/0022-2836(70)90337-2.
10
Restriction analysis of the nontranscribed spacers of Xenopus laevis ribosomal DNA.非洲爪蟾核糖体DNA非转录间隔区的限制性分析
Cell. 1977 Jul;11(3):599-607. doi: 10.1016/0092-8674(77)90077-0.

非洲爪蟾核糖体DNA中外转录间隔区的核苷酸序列：18S核糖体RNA 5'和3'末端侧翼的序列不互补。

Nucleotide sequence of an external transcribed spacer in Xenopus laevis rDNA: sequences flanking the 5' and 3' ends of 18S rRNA are non-complementary.

作者信息

Maden B E, Moss M, Salim M

出版信息

Nucleic Acids Res. 1982 Apr 10;10(7):2387-98. doi: 10.1093/nar/10.7.2387.

DOI:10.1093/nar/10.7.2387
PMID:6283480
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320617/
Abstract

We have sequenced the external transcribed spacer (ETS) of a ribosomal transcription unit from Xenopus laevis, together with sections of the preceding non-transcribed spacer. Our analysis was carried out on the same cloned transcription unit as that from which the internal transcribed spacers (ITS) were previously sequenced. The ETS is approximately 712 nucleotides long and, like the ITS regions, is generally very rich in C plus G. Features of the sequence include an excess of oligo-C tracts over oligo-G tracts and a tract of 37 nucleotides consisting almost entirely of G and A residues. Parts of the sequence can give rise to stable internal secondary structures. However, in contrast to Escherichia coli, there is no potential for major base-pairing between the 18S flanking regions of the ETS and ITS. Further findings are that there are no initiation (ATG) codons in the ETS and that, as in other X.laevis rDNA cloned units, the sequence preceding the ETS is duplicated, with a few changes, in the "Bam island" sequence of the non-transcribed spacer.

摘要

我们对非洲爪蟾核糖体转录单位的外部转录间隔区（ETS）以及之前的非转录间隔区片段进行了测序。我们的分析是在与之前对内部转录间隔区（ITS）进行测序的同一克隆转录单位上进行的。ETS大约有712个核苷酸长，并且与ITS区域一样，通常富含C加G。该序列的特征包括寡聚C序列多于寡聚G序列，以及一段几乎完全由G和A残基组成的37个核苷酸的序列。该序列的部分区域可形成稳定的内部二级结构。然而，与大肠杆菌不同的是，ETS的18S侧翼区域与ITS之间不存在主要碱基配对的可能性。进一步的发现是，ETS中没有起始（ATG）密码子，并且与其他非洲爪蟾rDNA克隆单位一样，ETS之前的序列在非转录间隔区的“Bam岛”序列中被复制，但有一些变化。