Lee P P, Buehring G C
J Natl Cancer Inst. 1981 Dec;67(6):1231-7.
Local IgA levels in 36 normal, 92 nonmalignant atypical, and 47 malignant human breast tissue specimens were analyzed by three methods: direct immunofluorescence, modified radioimmunoassay for IgA in cryostat sections, and the Immuno-Fluor (Bio-Rad Laboratories, Richmond, Calif.) assay for the cell surface IgA in tissue eluates. The proportion of epithelium in each sample was estimated microscopically. For malignant specimens the proportion of epithelial cells was significantly higher than that for nonmalignant specimens. The immunofluorescence test showed no qualitative difference in epithelial IgA content between normal and nonmalignant atypical tissues, but the frequency of positive specimens was markedly lower in malignant tissues. The modified radioimmunoassay and the Immuno-Fluor assay indicated little or no difference in IgA concentration per tissue weight among normal, nonmalignant atypical, and malignant breast tissues. However, when the data were corrected for proportion of glandular epithelium, nonmalignant atypical tissues had less IgA per cell than normal tissues, and malignant mammary carcinomas had significantly less IgA per cell than both normal and nonmalignant atypical tissues.
采用三种方法对36份正常、92份非恶性非典型和47份恶性人乳腺组织标本中的局部IgA水平进行了分析：直接免疫荧光法、冷冻切片中IgA的改良放射免疫测定法以及组织洗脱液中细胞表面IgA的免疫荧光（伯乐公司，加利福尼亚州里士满）测定法。通过显微镜估算每个样本中上皮组织的比例。对于恶性标本，上皮细胞的比例显著高于非恶性标本。免疫荧光试验显示，正常组织与非恶性非典型组织之间上皮IgA含量无定性差异，但恶性组织中阳性标本的频率明显较低。改良放射免疫测定法和免疫荧光测定法表明，正常、非恶性非典型和恶性乳腺组织每单位组织重量的IgA浓度几乎没有差异。然而，当数据根据腺上皮比例进行校正时，非恶性非典型组织每细胞的IgA含量低于正常组织，恶性乳腺癌每细胞的IgA含量明显低于正常组织和非恶性非典型组织。
