GR小鼠的内源性前病毒型小鼠乳腺肿瘤病毒基因并不相同，只有一个与外源性病毒相对应。
The endogenous proviral mouse mammary tumor virus genes of the GR mouse are not identical and only one corresponds to the exogenous virus.
作者信息
Herrlich P, Hynes N E, Ponta H, Rahmsdorf U, Kennedy N, Groner B
出版信息
Nucleic Acids Res. 1981 Oct 10;9(19):4981-95. doi: 10.1093/nar/9.19.4981.
The endogenous proviral copies of mouse mammary tumor virus (MMTV) were selected from a gene library of GR mouse DNA. We obtained five different lambda. MMTV recombinant clones. Four of them correspond to the 3' Eco RI fragments of the endogenous proviruses an one comprises an intact MMTV provirus with 2 to 3 kb of flanking mouse genomic DNA. Heteroduplex formation followed by S1 digestion under stringent conditions shows that there is nucleotide sequence heterology among the cloned endogenous proviral copies. Only one endogenous proviral copy, associated with the mtv-2 locus, was found to be totally homologous to the exogenous proviral DNA.
从小鼠乳腺肿瘤病毒（MMTV）的内源性前病毒拷贝是从GR小鼠DNA的基因文库中筛选出来的。我们获得了五个不同的λMMTV重组克隆。其中四个对应于内源性前病毒的3'Eco RI片段，一个包含一个完整的MMTV前病毒以及2至3 kb的侧翼小鼠基因组DNA。在严格条件下进行异源双链体形成后用S1酶切表明，克隆的内源性前病毒拷贝之间存在核苷酸序列异质性。仅发现一个与mtv-2位点相关的内源性前病毒拷贝与外源性前病毒DNA完全同源。
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