Quade K, Saule S, Stéhelin D, Kitchener G, Hayman M J
Imperial Cancer Reearch Fund, Lincoln's Inn Fields, P. 0. Box 123, London WC2A 3PX, England.
Virology. 1981 Dec;115(2):322-33. doi: 10.1016/0042-6822(81)90114-8.
Morphological revertants of the avian erythroblastosis virus (AEV)-transformed rat cell line ATla were isolated and characterised. The revertants are similar to the uninfected parental rat cell line in that they have regained an organized cytoskeleton and they are no longer capable of anchorage-independent growth. The pattern of integrated viral DNA in the revertants is indistinguishable from that of the transformed parent. However, the revertants do not express the integrated viral genome at either the mRNA or protein level. Phenotypic reversion thus is probably .due to reduced transcription of the AEV-transforming gene below a threshold necessary to induce morphological transformation.
分离并鉴定了禽成红细胞增多症病毒（AEV）转化的大鼠细胞系ATla的形态学回复突变体。这些回复突变体与未感染的亲代大鼠细胞系相似，因为它们重新获得了有组织的细胞骨架，并且不再能够进行不依赖贴壁的生长。回复突变体中整合病毒DNA的模式与转化亲本的模式无法区分。然而，回复突变体在mRNA或蛋白质水平上均不表达整合的病毒基因组。因此，表型回复可能是由于AEV转化基因的转录减少至低于诱导形态转化所需的阈值。
