Hansen J N, Konkel D A, Leder P
J Biol Chem. 1982 Jan 25;257(2):1048-52.
We have determined the nucleotide sequence of an embryonic beta-globin gene of the Balb/c mouse. It possesses structural features typical of known expressed beta-globins, including 5'-untranslated region, potential capping site, initiation and terminator codons, poly(A) addition signal, splicing signals, and intervening sequences. There is a striking 15-base homology between the putative RNA polymerase binding site of this gene and the corresponding region of a human embryonic beta-gene which may be important in the control of expression during embryonic development. The sequence of the gene predicts the sequence of the entire y2 protein, which has not previously been available. As expected from evolutionary considerations, it is more homologous to human embryonic beta-globin than to mouse adult beta-globin.
我们已经测定了Balb/c小鼠胚胎β-珠蛋白基因的核苷酸序列。它具有已知表达的β-珠蛋白典型的结构特征，包括5'-非翻译区、潜在的加帽位点、起始和终止密码子、聚腺苷酸添加信号、剪接信号和间隔序列。该基因假定的RNA聚合酶结合位点与人类胚胎β-基因的相应区域之间存在显著的15个碱基的同源性，这在胚胎发育过程中对表达的控制可能很重要。该基因的序列预测了整个γ2蛋白的序列，而此前该序列尚未获得。从进化的角度来看，正如预期的那样，它与人胚胎β-珠蛋白的同源性高于与小鼠成年β-珠蛋白的同源性。
