Tilghman S M, Tiemeier D C, Seidman J G, Peterlin B M, Sullivan M, Maizel J V, Leder P
Proc Natl Acad Sci U S A. 1978 Feb;75(2):725-9. doi: 10.1073/pnas.75.2.725.
The unusual electron microscopic appearance of a hybrid formed between 9S mouse beta-globin mRNA and its corresponding cloned gene segment is caused by at least one, and possibly two, intervening sequences of DNA that interrupt the mouse beta-globin gene. Such an interpretation is consistent with a paradoxical restriction site pattern previously noted in this gene and with the nucleotide sequence of that portion of the gene that spans both structural and intervening sequences. The large intervening sequence, approximately 550 base pairs in length, occurs in the structural globin sequence and immediately follows the beta-globin codon corresponding to amino acid 104. A smaller, putative intervening sequence is located close to the 5' end of the beta-globin-coding sequence but may reside beyond its initiation codon. The beta-globin gene thus appears to be encoded in two, and possibly three, discontinuous segments.
9S小鼠β-珠蛋白mRNA与其相应的克隆基因片段形成的杂种的异常电子显微镜外观是由至少一个、可能两个中断小鼠β-珠蛋白基因的DNA间隔序列引起的。这种解释与该基因先前观察到的矛盾限制酶切位点模式以及跨越结构序列和间隔序列的该基因部分的核苷酸序列一致。大的间隔序列长度约为550个碱基对，出现在结构珠蛋白序列中，并紧跟对应于第104位氨基酸的β-珠蛋白密码子之后。一个较小的假定间隔序列位于β-珠蛋白编码序列的5'端附近，但可能位于其起始密码子之外。因此，β-珠蛋白基因似乎由两个、可能三个不连续的片段编码。
