Kitts P, Symington L, Burke M, Reed R, Sherratt D
Proc Natl Acad Sci U S A. 1982 Jan;79(1):46-50. doi: 10.1073/pnas.79.1.46.
Cointegrate DNA molecules containing two copies of a transposable element appear to be intermediates in the transposition process. These structures are resolved by site-specific recombination to yield the normal end products of transposition. The transposable element gamma delta (Tn1000) synthesizes a product interchangeable with the Tn1/3tnpR protein in promoting Tn1/3 site-specific recombination. These data support the hypothesis that cointegrates containing directly repeated copies of Tn1/3 are obligatory intermediates in interreplicon transposition of Tn1/3. In addition, we show here that the reaction is independent of the element-encoded tnpA gene product. Tn501, which specifies mercury resistance, also produces cointegrates as intermediates in interreplicon transposition. The appearance of Tn501-specified recombination activity that can act on these cointegrates requires growth of cells in the presence of Hg2+.
含有两个转座元件拷贝的共整合DNA分子似乎是转座过程中的中间体。这些结构通过位点特异性重组得以分解，从而产生转座的正常终产物。转座元件γδ（Tn1000）在促进Tn1/3位点特异性重组时，合成一种可与Tn1/3tnpR蛋白互换的产物。这些数据支持这样一种假说，即含有Tn1/3直接重复拷贝的共整合体是Tn1/3在复制子间转座的必需中间体。此外，我们在此表明该反应独立于元件编码的tnpA基因产物。赋予汞抗性的Tn501在复制子间转座时也会产生共整合体作为中间体。能够作用于这些共整合体的Tn501特异性重组活性的出现需要细胞在Hg2+存在的情况下生长。
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