Institut de Biologie Moléculaire et Cellulaire, Université Louis Pasteur, 15 Rue Descartes, 67084, Strasbourg Cedex, France.
Plant Mol Biol. 1985 Nov;5(6):347-51. doi: 10.1007/BF00037555.
Two bean mitochondrial tRNAs(Tyr) purified by RPC-5 chromatography and two-dimensional gel electrophoresis have been sequenced using post-labeling techniques. These two tRNAs only differ by three post-transcriptional modifications in the D-loop. They have a large variable loop and therefore resemble prokaryotic tRNAs(Tyr) rather than eukaryotic cytoplasmic tRNAs(Tyr).
经 RPC-5 层析和二维凝胶电泳纯化的两种豆科植物线粒体 tRNA(Tyr),采用标记后技术进行了测序。这两种 tRNA 在 D 环中仅差三个转录后修饰。它们具有较大的可变环,因此与真核细胞质 tRNA(Tyr)不同,更类似于原核 tRNA(Tyr)。