Fauci A S, Pratt K R
J Exp Med. 1976 Sep 1;144(3):674-84. doi: 10.1084/jem.144.3.674.
A model for the detection of single cell antibody production by human tonsillar lymphocytes after stimulation with either sheep red blood cells (SRBC) or polyclonal B-cell activators has been described. The culture system is a modified Mishell-Dutton technique with certain critical factors identified. The assay is a sensitive and resproducible hemolysis-in-gel system employing an ultra-thin layer gel technique measuring plaque-forming cells (PFC) against SRBC targets. Several factors essential for optimal responses are described, but the critical feature of the culture system is the use of selected lots of human AB serum supplements which are extensively absorbed with SRBC. This removes a blocking factor present in most human serum which suppresses the B-cell response to SRBC targets after stimulation with either SRBC or several polyclonal B-cell activators. In addition, absorption of serum with SRBC eliminates the presence of artifactual plaques. Background PFC are extremely low and stimulated cultures show significant and reproducible responses. These studies provide a simple, sensitive, and reproducible model for probing the complex events associated with activation of human B lymphocytes.
已经描述了一种用于检测人扁桃体淋巴细胞在用绵羊红细胞（SRBC）或多克隆B细胞激活剂刺激后产生单细胞抗体的模型。培养系统是一种改良的米舍尔-达顿技术，确定了某些关键因素。该测定是一种灵敏且可重复的凝胶内溶血系统，采用超薄层凝胶技术测量针对SRBC靶标的空斑形成细胞（PFC）。描述了几个实现最佳反应所必需的因素，但培养系统的关键特征是使用经过大量SRBC广泛吸收的特定批次的人AB血清补充剂。这消除了大多数人血清中存在的一种阻断因子，该因子在用SRBC或几种多克隆B细胞激活剂刺激后会抑制B细胞对SRBC靶标的反应。此外，用SRBC吸收血清可消除人为空斑的存在。背景PFC极低，刺激后的培养物显示出显著且可重复的反应。这些研究为探究与人类B淋巴细胞激活相关的复杂事件提供了一个简单、灵敏且可重复的模型。
