Dutton R W
J Exp Med. 1972 Dec 1;136(6):1445-60. doi: 10.1084/jem.136.6.1445.
The presence of concanavalin A (Con A) inhibits the immune response of mouse spleen cell suspensions to erythrocyte antigens, stimulates the incorporation of tritiated thymidine, and increases cell recovery. Con A also restores the depressed response of cell preparations treated to remove thymus-derived cells. The dose-response curve for all four effects shows peak activity at 2 microg/ml. The depressed in vitro response of spleen cell suspensions from adult thymectomized, irradiated, bone marrow-restored mice is also restored by Con A. Here the dose-response curve is quite different with activity over a much wider range of concentration. The restoration of thymus-derived cell-depleted cultures by Con A is inhibited by the addition of untreated, unirradiated, mouse spleen cell suspensions, but is not inhibited by untreated, irradiated cells. Small numbers of spleen cells that have been preincubated with Con A and washed will inhibit the response of fresh, untreated cells to antigen. If the mouse spleen cell suspensions are incubated for 24 hr before the addition of Con A, the response to antigen is no longer inhibited but is stimulated instead. The data are compatible with the hypothesis that there are at least two cell targets for the action of Con A. One cell, that mediates the inhibitor effect, is a short-lived, radiosensitive, thymus-derived cell. The other cell, that mediates the stimulating effect, cannot be identified from the data presented here but may also be of thymus origin on the basis of studies by other investigators.
伴刀豆球蛋白A（Con A）的存在会抑制小鼠脾细胞悬液对红细胞抗原的免疫反应，刺激氚标记胸腺嘧啶核苷的掺入，并增加细胞回收率。Con A还能恢复经处理去除胸腺来源细胞的细胞制剂的抑制反应。所有这四种效应的剂量反应曲线在2微克/毫升时显示出峰值活性。Con A也能恢复成年去胸腺、受照射、骨髓重建小鼠脾细胞悬液在体外的抑制反应。在这里，剂量反应曲线有很大不同，在更宽的浓度范围内都有活性。添加未处理、未受照射的小鼠脾细胞悬液会抑制Con A对胸腺来源细胞耗竭培养物的恢复作用，但未处理、受照射的细胞则不会。少量预先与Con A孵育并洗涤过的脾细胞会抑制新鲜未处理细胞对抗原的反应。如果在添加Con A之前将小鼠脾细胞悬液孵育24小时，对抗原的反应不再被抑制，反而受到刺激。这些数据与以下假设相符：Con A的作用至少有两个细胞靶点。一个介导抑制作用的细胞是寿命短、对辐射敏感的胸腺来源细胞。另一个介导刺激作用的细胞无法从这里给出的数据中确定，但根据其他研究者的研究，可能也起源于胸腺。
